Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice
Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice
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肠道微生物群、宿主遗传学和饮食之间的相互作用与小鼠代谢综合征的发生相关
DOI：
10.1038/ismej.2009.112
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发表时间：
2010-02-01
期刊：
影响因子：
11
通讯作者：
Zhao, Liping
中科院分区：
文献类型：
--
作者：
Zhang, Chenhong;Zhang, Menghui;Zhao, Liping
遗传变异和饮食破坏的肠道微生物群都可能使动物容易患上代谢综合征（MS）。这项研究评估了宿主遗传学和饮食在塑造肠道微生物群和调节小鼠MS相关表型方面的相对贡献。将Apoa-I基因敲除小鼠与其野生型（Wt）对应小鼠一起喂饲高脂饮食（HFD）或正常食物（NC）25周，所述Apoa-I基因敲除小鼠具有糖耐量受损（IGT）和体脂增加。使用16S rRNA基因的DNA指纹和条形码焦磷酸测序来分析肠道微生物群结构，并通过偏最小二乘判别分析来确定与MS发展相关的关键群体变化。饮食变化解释了肠道微生物群总结构变化的57%，而基因突变占不到12%。相对于健康的Wt/NC喂养的动物，IGT的所有三个组具有显著不同的肠道微生物群。在所有的，65个物种水平的MS类型被确定为关键成员的饮食，基因型和MS表型的变化有差异的反应。最值得注意的是，肠道屏障保护双歧杆菌属。几乎不存在于所有动物的HFD，无论基因型。硫酸盐还原，产生内毒素的细菌的家庭，脱硫弧菌科，增强在所有动物IGT，最显着的Wt/HFD组，其中有最高的热量摄入和最严重的MS表型。因此，饮食在形成肠道微生物群中具有主导作用，并且一些关键群体的变化可能将野生型动物的肠道微生物群转化为与MS发展相关的病原体样实体，尽管具有完整的宿主基因组。
Both genetic variations and diet-disrupted gut microbiota can predispose animals to metabolic syndromes (MS). This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota and modulating MS-relevant phenotypes in mice. Together with its wild-type (Wt) counterpart, theApoa-I knockout mouse, which has impaired glucose tolerance (IGT) and increased body fat, was fed a high-fat diet (HFD) or normal chow (NC) diet for 25 weeks. DNA fingerprinting and bar-coded pyrosequencing of 16S rRNA genes were used to profile gut microbiota structures and to identify the key population changes relevant to MS development by Partial Least Square Discriminate Analysis. Diet changes explained 57% of the total structural variation in gut microbiota, whereas genetic mutation accounted for no more than 12%. All three groups with IGT had significantly different gut microbiota relative to healthy Wt/NC-fed animals. In all, 65 species-level phylotypes were identified as key members with differential responses to changes in diet, genotype and MS phenotype. Most notably, gut barrier-protectingBifidobacteriumspp. were nearly absent in all animals on HFD, regardless of genotype. Sulphate-reducing, endotoxin-producing bacteria of the family,Desulfovibrionaceae, were enhanced in all animals with IGT, most significantly in the Wt/HFD group, which had the highest calorie intake and the most serious MS phenotypes. Thus, diet has a dominating role in shaping gut microbiota and changes of some key populations may transform the gut microbiota of Wt animals into a pathogen-like entity relevant to development of MS, despite a complete host genome.