Synovial fluid nitric oxide levels in patients with knee osteoarthritis
Synovial fluid nitric oxide levels in patients with knee osteoarthritis
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DOI：
10.1007/s10067-003-0761-y
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发表时间：
2003-12-01
影响因子：
3.4
通讯作者：
Öncel, A
中科院分区：
文献类型：
--
作者：
Karan, A;Karan, MA;Öncel, A
一氧化氮(NO)在炎性关节病中起重要作用。本研究调查了膝关节原发性骨关节炎(OA)患者滑液和血浆中的NO水平。27例原发膝骨性关节炎患者和13例正常对照被纳入研究。用Griess法测定滑液和血浆中硝酸盐/亚硝酸盐水平，用双抗体夹心免疫法测定血清中白细胞介素1β(IL-1β)水平。我们发现原发性膝关节骨性关节炎患者滑液硝酸盐/亚硝酸盐水平显著高于对照组(50.26+/-23.63MU/L vs32.49+/-10.05MU/L，p=0.002)和血浆硝酸盐/亚硝酸盐水平(57.06+/-23.32MU/L vs39.98+/-16.36MU/L，P=0.012)。血浆和滑液中IL-1β浓度在研究组和对照组之间无差异。这些结果可能被认为是NO可能是导致膝关节原发性骨关节炎软骨破坏的因素之一。
Nitric oxide (NO) has an important role in the inflammatory arthropathies. This study investigated NO levels in the synovial fluid and plasma of patients with primary osteoarthritis (OA) of the knee. Twenty-seven cases with primary knee OA and 13 controls were recruited for the study. Nitrate/nitrite levels of synovial fluid and plasma were measured by Griess reaction, and interleukin-1beta (IL-1beta) levels were measured quantitatively by a sandwich immunoassay technique. We found a significant increase in the synovial fluid nitrate/nitrite levels in cases with primary OA of the knee compared to controls (50.26+/-23.63 mug/l vs 32.49+/-10.05 mug/l, p=0.002) as well as increased plasma nitrate/nitrite levels (57.06+/-23.32 mug/l vs 39.98+/-16.36 mug/l, p=0.012). There was no difference in plasma and synovial fluid IL-1beta concentrations between the study and control groups. These results may be considered as supporting evidence that NO might be one of the factors responsible for cartilage destruction in primary osteoarthritis of the knee.