Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys
Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys
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DOI：
10.1007/s00251-006-0101-x
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发表时间：
2006-07-01
期刊：
影响因子：
3.2
通讯作者：
Parra-Lopez, Carlos
中科院分区：
文献类型：
--
作者：
Baquero, Juan E.;Miranda, Santiago;Parra-Lopez, Carlos
Aotus猴在疟疾候选疫苗的临床前研究中具有重要价值。这种灵长类动物的免疫系统的几个组成部分已被研究，它们与人类的免疫系统非常相似。克隆和测序研究已经揭示了Aotus MHC-DRB中广泛的序列多态性，其与几种人类等位基因谱系具有非常高的相似性，将至少9种不同的MHC-DRB谱系分组。由于肽疫苗在该动物模型中的效力可能受到外显子2 MHC-DRB多态性的强烈影响，因此需要针对三种Aotus（Aotus naneymaae、Aotus vociferans和Aotus nigriceps）的可靠且快速的MHC-DRB分型方法的可用性。这里使用参考链构象分析（RSCA）来区分独特的Aotus MHC-DRB序列的迁移率，使用五个荧光标记的参考，证明对于解析密切相关的序列、确定特定猴子中转录的序列数量及其身份非常有用。RSCA方法在鉴定Aotus MHC-DRB序列方面的可靠性将有助于评价个体对疫苗的反应性，并促进与感染因子或自身免疫性疾病的易感性/抗性相关的研究，Aotus猴可被认为是适当的动物模型。
The Aotus monkey has been of great value in the pre-clinical study of malaria vaccine candidates. Several components of this primate's immune system have been studied and they display great similarity to their human counterparts. Cloning and sequencing studies have revealed extensive sequence polymorphisms in Aotus MHC-DRB with very high similarities to several human allelic lineages, grouping at least nine distinct MHC-DRB lineages. As the efficacy of peptide vaccines in this animal model may be strongly influenced by exon 2 MHC-DRB polymorphism, the availability of a reliable and rapid MHC-DRB typing method for three species of Aotus (Aotus naneymaae, Aotus vociferans and Aotus nigriceps) is necessary. Reference strand conformational analysis (RSCA) was used here for differentiating the distinctive Aotus MHC-DRB sequences' mobility using five fluorescently labelled references proved to be very useful for resolving closely related sequences, establishing the number of sequences transcribed in a particular monkey and their identity. The RSCA method's reliability in terms of identifying Aotus MHC-DRB sequences will facilitate evaluating individual responsiveness to vaccines and prompt studies associating susceptibility/resistance to infectious agents or auto-immune disease, for which Aotus monkeys may be considered to be an appropriate animal model.