FcγRIIIA-158V and rheumatoid arthritis:: a confirmation study
FcγRIIIA-158V and rheumatoid arthritis:: a confirmation study
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DOI：
10.1093/rheumatology/keg169
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发表时间：
2003-04-01
期刊：
影响因子：
5.5
通讯作者：
Isaacs, JD
中科院分区：
文献类型：
--
作者：
Morgan, AW;Keyte, VH;Isaacs, JD
目标.目的：建立Fc γ RIIIA-158 V/F多态性基因分型的方法，并证实Fc γ RIIIA-158 V等位基因与类风湿性关节炎（RA）之间的关联。该等位基因关联研究检查了Fc γ RIIIA-158 V/F多态性与RA的关联。采用一种新的单链构象多态性分析方法对来自英国的828名RA患者和581名对照进行基因分型。Fc γ RIIIA-158 V等位基因与RA（P=0.02）和结节（P=0.04）相关。该高亲和力等位基因纯合子个体患RA（OR 1.53，95% CI 1.08-2.18）和结节发生（OR 2.20，95% CI 1.20-4.01）的风险显著增加。没有证据表明与共享表位的相互作用。我们已经开发了一种新的检测方法对Fc γ RIIIA-158 F/V多态性进行基因分型，并证实Fc γ RIIIA-158 V等位基因的纯合性与UK高加索人RA相关，特别是在那些患有结节的个体中，这表明Fc γ RIIIA可能在确定疾病严重程度或结节本身的发展中发挥作用。
Objectives. To develop a robust assay for genotyping the FcgammaRIIIA-158V/F polymorphism and to confirm the putative association between the FcgammaRIIIA-158V allele and rheumatoid arthritis (RA).Methods. This allelic association study examined the FcgammaRIIIA-158V/F polymorphism for association with RA. A novel single-stranded conformational polymorphism assay was used to genotype 828 RA patients and 581 controls from the UK.Results. The FcgammaRIIIA-158V allele was associated with both RA (P=0.02) and nodules (P=0.04). Individuals homozygous for this higher affinity allele had a significantly increased risk of RA (OR 1.53, 95% CI 1.08-2.18) and the development of nodules (OR 2.20, 95% CI 1.20-4.01). There was no evidence of an interaction with the shared epitope.Conclusions. We have developed a novel assay to genotype the FcgammaRIIIA-158F/V polymorphism and confirmed that homozygosity for the FcgammaRIIIA-158V allele is associated with UK Caucasian RA, particularly in those individuals with nodules, suggesting FcgammaRIIIA may play a role in determining disease severity or in the development of nodules per se.