Isolation of two isoforms of a novel 15-kDa protein from rabbit polymorphonuclear leukocytes that modulate the antibacterial actions of other leukocyte proteins.
Isolation of two isoforms of a novel 15-kDa protein from rabbit polymorphonuclear leukocytes that modulate the antibacterial actions of other leukocyte proteins.
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发表时间：
1990-09
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通讯作者：
C. E. Ooi;J. Weiss;O. Levy;P. Elsbach
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作者：
C. E. Ooi;J. Weiss;O. Levy;P. Elsbach
我们最近报道了利用高效杀菌/通透性增加蛋白(BPI)的高选择性和可逆性结合到靶向革兰氏阴性菌(EscherichiaColi)的方法，从人多形核白细胞(PMN)的粗提液中分离出BPI。我们现在报告使用相同的程序从兔PMN中纯化BPI和一种新的15 kDa物种，该物种由两个几乎相同的异构体组成。这些15 kDa的蛋白质本身没有明显的抗菌活性。然而，一种异构体(P15A)对兔和人BPI的早期抗菌作用有很强的增强作用，而另一种(P15B)亚型对人和兔BPI的早期抗菌作用有微弱的增强作用。这两种异构体都抑制BPI的晚期致死作用。而这种增强作用是BPI所特有的，而另一种PMN颗粒的抗菌蛋白天青素也有抑制作用。因此，我们已经在兔PMN中确定了一种以前未被识别的15 kDa蛋白质物种，它可能在吞噬过程中调节BPI(和其他颗粒蛋白)的抗菌效果。
We have recently reported the use of the highly selective and reversible binding of the potent bactericidal/permeability-increasing protein (BPI) to target Gram-negative bacteria (Escherichia coli) for its isolation from crude extracts of human polymorphonuclear leukocytes (PMN). We now report the use of the same procedure for the purification from rabbit PMN of BPI and also of a novel 15-kDa species that consists of two nearly identical isoforms. These 15-kDa proteins have no demonstrable antibacterial activities by themselves. However, one isoform (p15A) potentiates strongly and the other (p15B) weakly the early antibacterial effects of both rabbit and human BPI. Both isoforms inhibit the late lethal action of BPI. Whereas the potentiating effect is specific for BPI the inhibitory effect is seen also with another antibacterial protein of PMN granules, azurocidin. Thus, we have identified in rabbit PMN a previously unrecognized 15-kDa protein species that may modulate during phagocytosis the antimicrobial effects of BPI (and other granule proteins).