Vanin-1, a novel GPI-linked perivascular molecule involved in thymus homing
Vanin-1, a novel GPI-linked perivascular molecule involved in thymus homing
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DOI：
10.1016/s1074-7613(00)80496-3
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发表时间：
1996-11-01
期刊：
影响因子：
32.4
通讯作者：
Naquet, P
中科院分区：
文献类型：
--
作者：
AurrandLions, M;Galland, F;Naquet, P
造血前细胞向胸腺的迁移依赖于胸腺血管周围基质细胞表达的一种名为Vanin-1的新分子。抗vanin -1抗体在体外可阻断前t细胞与胸腺切片的结合，体内胸腺皮质血管周围骨髓细胞的积累，以及长期的胸腺再生。因此，它干扰了造血前体细胞的进入，而不是分化。Vanin-1基因编码gpi锚定的70 kDa蛋白，该蛋白仅与人类生物素酶具有同源性。用Vanin-1 cDNA转染胸腺基质细胞增强胸腺细胞的体外粘附。这些数据表明，在生理、非炎症条件下，vanin-1调节胸腺归巢的晚期粘附步骤。
Migration of hematopoietic precursor cells to the thymus is shown to depend upon a novel molecule called Vanin-1 expressed by perivascular thymic stromal cells. An anti-Vanin-1 antibody blocks the binding of pro-T cells to thymic sections in vitro, the in vivo accumulation of bone marrow cells around cortical thymic vessels, and long-term thymic regeneration. Thus, it interferes with the entry, and not the differentiation, of hematopoietic precursor cells. The Vanin-1 gene codes for a GPI-anchored 70 kDa protein that shows homology only with human biotinidase. Transfection of thymic stromal cells with the Vanin-1 cDNA enhances thymocyte adhesion in vitro. These data suggest that vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions.