Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer
Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer
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DOI：
10.1038/labinvest.3700146
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发表时间：
2004-10-01
影响因子：
5
通讯作者：
Nagawa, H
中科院分区：
文献类型：
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作者：
Shida, D;Watanabe, T;Nagawa, H
溶血磷脂酸（LPA）是一种简单的生物活性磷脂，对各种细胞具有不同的作用，与三种G蛋白偶联的跨膜受体LPA 1，LPA 2和LPA 3相互作用。这些LPA受体在各种肿瘤中的表达模式和功能尚未得到充分研究，除了卵巢癌。为了评估LPA受体在人结直肠癌和正常粘膜中的表达谱，我们使用实时逆转录-聚合酶链反应（RT-PCR），并测量26例结直肠癌和16例相应的正常组织样本中LPA 1，LPA 2和LPA 3信使RNA（mRNA）的表达水平。正常上皮表达LPA 1和LPA 2 mRNA在相似的水平。相比之下，结直肠癌表达LPA 1 mRNA的水平显著较低（0.3倍; P
Lysophosphatidic acid (LPA) is a simple bioactive phospholipid with diverse effects on various cells, that interacts with three G protein-coupled transmembrane receptors, LPA1, LPA2, and LPA3. The expression pattern and functions of these LPA receptors in various tumors have not been fully examined, except in ovarian cancer. To evaluate the LPA receptor expression profile in human colorectal cancer and in normal mucosa, we used real-time reverse transcription-polymerase chain reaction (RT-PCR) and measured the expression levels of LPA1, LPA2, and LPA3 messenger RNA (mRNA) in 26 colorectal cancers and 16 corresponding normal tissue samples. Normal epithelium expressed both LPA1 and LPA2 mRNA at similar levels. In comparison, colorectal cancers expressed LPA1 mRNA at a significantly lower level (0.3-fold; P