Developmental changes in the astrocytic response to lateral olfactory tract section.
Developmental changes in the astrocytic response to lateral olfactory tract section.
复制标题
星形胶质细胞对侧嗅道部分反应的发育变化。
DOI：
10.1002/ar.1092150407
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Leonard,CM
中科院分区：
文献类型：
--
作者：
Sijbesma,H;Leonard,CM
When the lateral olfactory tract (LOT) of the golden hamster,Meso cricetus auratus, is transected in the first week of postnatal life, axons can grow back past the lesion and achieve functional reinnervation of caudal projection regions. In contrast, when the tract is sectioned after postnatal day 7 (P7), axons do not reinnervate regions caudal to the cut. The experiments reported here investigated whether regenerative failure after tract section in pups older than P7 is accompanied by developmental changes in the astrocytic response. LOT transections were performed at P3 and P9 and the glial reaction was observed at survival times ranging from 12 hr to 2 weeks. Immunocytochemistry with glial fibrillary acidic protein (GFAP) was employed for histological visualization of astrocytic reactivity.Staining for GFAP immunoreactivity showed an appreciable glial reaction after tract section at both P3 and P9, but the extent of astrocytic hypertrophy and proliferation of glial processes was considerably greater and more extensive after tract section at P9. Radial glial cells were observed 2 weeks after LOT transection at P3 but were absent after lesions made at P9.The results from this study suggest that the developmental loss of regenerative capacity after LOT transection may be related to maturational changes in the glial response. In particular, the presence of radial glial elements after P3 lesions could serve to establish a more favorable microenvironment for axonal elongation.
