A single session of intense exercise improves the inflammatory response in healthy sedentary women
A single session of intense exercise improves the inflammatory response in healthy sedentary women
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DOI：
10.1007/s13105-010-0052-4
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发表时间：
2011-03-01
影响因子：
3.4
通讯作者：
Ortega, Eduardo
中科院分区：
文献类型：
--
作者：
Jose Garcia, Juan;Bote, Elena;Ortega, Eduardo
体育活动有很大的能力调节免疫系统，包括炎症反应。然而，运动对女性炎症反应的影响很少被研究，尽管女性比男性更容易患慢性炎症性疾病。这项研究的目的是确定单次运动对久坐女性炎症反应的影响，评估中性粒细胞功能和炎症细胞因子的循环浓度。运动包括在自行车测力仪上进行一次骑行（1小时，以70%的最大摄氧量）。在基础状态和运动结束后立即采集血液样本。通过评估中性粒细胞对乳胶珠的吞噬能力和超氧阴离子（O-2(-)）产生所反映的氧依赖性杀微生物能力，研究了分离细胞的中性粒细胞功能。循环炎症细胞因子是用一种新的基于抗体的蛋白质微阵列方法测定的。ELISA法测定白细胞介素-8（一种刺激中性粒细胞的细胞因子）的循环浓度。运动增加了久坐女性中性粒细胞的吞噬能力和依赖氧的杀微生物能力。IL-2、IL-3、IL-5、IL-6、IL-7、IL-8、IL-10、IL-13、ifn - γ、tnf - α和- β、tgf - β、MCP-2和-3、MIG、G-CSF和GM-CSF均无变化。然而，运动后GRO和MCP-1的循环浓度升高，促炎细胞因子RANTES的循环浓度降低。运动改善中性粒细胞功能，可能介导，至少部分，由GRO（一种有效的中性粒细胞激活剂），而不是由IL-8。中性粒细胞功能的刺激似乎没有伴随着任何有害的全身炎症反应，因为没有观察到主要促炎细胞因子的变化，RANTES也有所下降。
Physical activity has a great capacity for modulating the immune system, including the inflammatory response. However, the effects of exercise on the inflammatory response have rarely been studied in women, even though women are more susceptible than men to chronic inflammatory diseases. The aim of this study was to ascertain the effect of single sessions of exercise on the inflammatory response of sedentary women, evaluating neutrophil function and circulating concentrations of inflammatory cytokines. Exercise consisted of one session of cycling (1 h at similar to 70% of VO2 max) on a cycle ergometer. Blood samples were taken in the basal state and immediately after the exercise session. Neutrophil function was studied on isolated cells by evaluating their phagocytic capacity against latex beads and their oxygen-dependent microbicidal capacity as reflected in the superoxide anion (O-2 (-)) production. Circulating inflammatory cytokines were determined using a novel antibody-based protein micro-array method. The circulating concentration of IL-8 (a stimulatory cytokine for neutrophils) was also determined by ELISA. Exercise increased the phagocytic and the oxygen-dependent microbicidal capacities of neutrophils in the sedentary women. No variations were found in IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, IFN-gamma, TNF-alpha and -beta, TGF-beta, MCP-2 and -3, MIG, G-CSF, and GM-CSF. However, while the circulating concentrations of GRO and MCP-1 increased after exercise, there was a decrease in that of RANTES, a pro-inflammatory cytokine. Exercise improves neutrophil function, possibly mediated, at least partially, by GRO (a potent neutrophil activator) but not by IL-8. This stimulation of neutrophil function does not seem to be accompanied by any harmful systemic inflammatory response since no changes in the main pro-inflammatory cytokines were observed, and there was a decrease in RANTES.