Assessment of JC polyoma virus in colon neoplasms
Assessment of JC polyoma virus in colon neoplasms
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DOI：
10.1007/s10350-004-0737-2
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发表时间：
2005-01-01
影响因子：
3.9
通讯作者：
Lazaris, AC
中科院分区：
文献类型：
--
作者：
Theodoropoulos, G;Panoussopoulos, D;Lazaris, AC
目的：最近的研究资料强调JC多瘤病毒与结肠癌发生之间存在着触发性的联系。JC病毒的致瘤性归因于其Mad-1变体的T抗原。当另一个研究小组没有证实这种联系时，争议就出现了。本研究的目的是检测JC病毒在一系列的结肠肿瘤从希腊patients.METHODS：巢式聚合酶链反应检测JC病毒在80癌，25腺瘤标本大肠，和20例结肠镜活检标本正常患者无结直肠肿瘤。结果：JC多瘤病毒核苷酸序列在61%的结肠腺癌和60%的腺瘤中检测到，病毒载量为9 × 10（3）到20 × 10（3）拷贝/μ g DNA。35例阳性结肠腺癌标本中的邻近正常粘膜和对照组6例患者的正常粘膜的病毒载量较低（50-450拷贝/μ g DNA）。结论：JC多瘤病毒基因组存在于结肠肿瘤中。在腺瘤中检测到JC病毒，其病毒载量与恶性肿瘤相当，表明其在结肠癌发生的早期阶段的意义。考虑到其他已发表的数据，JC病毒感染结肠上皮可能是染色体和基因组不稳定性的主要候选者。
PURPOSE: Research data have recently emphasized an in triguing association of JC polyoma virus with colon carcinogenesis. Tumorigenicity of JC virus is attributed to the T-antigen of its Mad-1 variant. Controversy arose when another research group did not confirm this association. The purpose of this study was to detect JC virus in a series of colon neoplasms from Greek patients.METHODS: A nested polymerase chain reaction assay was used to detect JC virus in 80 cancerous, 25 adenomatous specimens of large bowel, and 20 colonoscopic biopsy samples from normal patients without colorectal neoplasia. Quantitation of JC virus DNA was performed by real-time polymerase chain reaction.RESULTS: JC polyoma virus nucleotide sequence was detected in 61 percent of colon adenocarcinomas and in 60 percent of adenomas, at a viral load of 9 x 10(3) to 20 x 10(3) copies/mug DNA. Adjacent normal mucosa in 35 positive colon adenocarcinoma specimens, and normal mucosa from six patients of the control group, had low viral loads (50-450 copies/mug DNA).CONCLUSIONS: JC polyoma virus genome is present in colon neoplasms. JC virus detection in adenomas at comparable viral loads to malignant tumors suggests its implication at early steps of colonic carcinogenesis. Taking into consideration other published data, infection of colonic epithelium with JC virus might be a prime candidate for a role in chromosomal and genomic instability.