The protective effect of fish n-3 fatty acids on cerebral ischemia in rat hippocampus
The protective effect of fish n-3 fatty acids on cerebral ischemia in rat hippocampus
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DOI：
10.1016/j.neuint.2006.11.005
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发表时间：
2007-02-01
影响因子：
4.2
通讯作者：
Yagmurca, Murat
中科院分区：
文献类型：
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作者：
Bas, Orhan;Songur, Ahmet;Yagmurca, Murat
活性氧自由基(ROS)参与了脑缺血再灌注损伤的发病机制。鱼n-3必需脂肪酸(EFA)含有二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)，具有抗氧化特性。DHA是脑膜磷脂的重要组成部分，对神经元功能的连续性是必需的。EPA可防止血小板聚集，并抑制花生四烯酸转化为血栓素A(2)和前列腺素。它们被认为是预防神经和神经精神疾病的保护剂。本研究观察了FISH n-3 EFA对脑I/R损伤大鼠海马区氧化-抗氧化系统和细胞凋亡的保护作用。脑缺血模型采用双侧颈总动脉结扎加降压45min，再灌流30min的方法，分别给予标准饲料(II组)或标准饲料+FISH n-3 EFA(Marincap1，0.4g/kg/d，灌胃)14d。手术结束后，处死大鼠，并立即取出它们的脑。测定左侧HE组织丙二醛(MDA)、一氧化氮(NO)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性，并用末端转移酶dUTP缺口末端标记法(JUNEL)计数右侧HE组织标本中的神经细胞凋亡数。另一方面，与II组大鼠相比，III组大鼠的CAT活性和NO水平降低。此外，与II组相比，III组大鼠海马神经元的凋亡数明显减少。本研究结果提示，FISH n-3 EFA可降低缺血大鼠海马区的氧化状态和细胞凋亡的变化。膳食补充n-3EFA可能有利于保护或改善缺血性脑血管疾病。(C)2006爱思唯尔有限公司。保留所有权利。
Reactive oxygen species (ROS) have been implicated in the pathogenesis of cerebral injury after ischemia-reperfusion (I/R). Fish n-3 essential fatty acids (EFA), contain eicosapentaenoic acids (EPA) and docosahexoenoic acids (DHA), exhibit antioxidant properties. DHA is an important component of brain membrane phospholipids and is necessary for the continuity of neuronal functions. EPA prevents platelet aggregation and inhibits the conversion of arachidonic acid into thromboxane A(2) and prostaglandins. They have been suggested to be protective agents against neurological and neuropsychiatric disorders. In this study, the neuroprotective effects of fish n-3 EFA on oxidant-antioxidant systems and number of apoptotic neurons of the hippocampal formation (HF) subjected to cerebral I/R injury was investigated in Sprague-Dawley rats.Six rats were used as control (Group I). CerebrEd ischemia was produced by occlusion of both the common carotid arteries combined with hypotension for 45 min, followed by reperfusion for 30 min, in rats either on a standard diet (Group II) or a standard diet plus fish n-3 EFA (Marincap (R), 0.4 g/kg/day, by gavage) for 14 days (Group III). At the end of procedures, the rats were sacrificed and their brains were removed immediately. The levels of malonedialdehyde (MDA) and nitric oxide (NO) and activities of superoxide dismutase (SOD) and catalase (CAT) were measured in left HE In addition, the number of apoptotic neurons was counted by terminal transferase dUTP nick end labelling JUNEL) assay in histological samples of the right HF.We found that SOD activities and MDA levels increased in Group III rats compared with Group II rats. On the other hand, CAT activities and NO levels were found to be decreased in Group III rats compared with Group II rats. Additionally, the number of apoptotic neurons was lower in Group III in comparison with Group II rats.The present findings suggest that fish n-3 EFA could decrease the oxidative status and apoptotic changes in ischemic rat hippocampal formation. Dietary supplementation of n-3 EFA may be beneficial to preserve or ameliorate ischernic cerebral vascular disease. (c) 2006 Elsevier Ltd. All rights reserved.