Multimodal Measurements of Brain Tissue Metabolism and Perfusion in a Neonatal Model of Hypoxic-Ischaemic Injury.
Multimodal Measurements of Brain Tissue Metabolism and Perfusion in a Neonatal Model of Hypoxic-Ischaemic Injury.
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新生儿缺氧缺血性损伤模型中脑组织代谢和灌注的多模式测量。
DOI：
10.1007/978-3-030-48238-1_32
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发表时间：
2021
影响因子：
--
通讯作者：
Bale G
中科院分区：
文献类型：
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作者：
Bale G
这是第一次使用宽带近红外光谱（bNIRS），扩散相关光谱（DCS），正电子发射断层扫描（PET）和磁共振波谱（MRS）对缺氧缺血（HI）脑损伤后脑组织代谢和灌注的多模式研究。在7头新生儿HI的临床前模型中，我们测量了脑组织饱和度（StO 2）、脑血流量（CBF）、脑氧代谢（CMRO 2）、细胞色素c氧化酶（oxCCO）的线粒体氧化态变化、脑葡萄糖代谢（CMRglc）和组织生化（Lac+Thr/tNAA）。基线时，在发生HI的仔猪（非对照组）中测量的参数为64 ± 6% StO 2、35 ± 11 ml/100 g/min CBF和2.0 ± 0.4 μmol/100 g/min CMRO 2。HI后测定的参数为68 ± 6% StO 2、35 ± 6 ml/100 g/min CBF、1.3 ± 0.1 μmol/100 g/min CMRO 2、0.4 ± 0.2 Lac+Thr/tNAA和9.5 ± 2.0 CMRglc。本研究证明了多模式设置的能力，询问HIE的病理生理学使用的光学方法，MRS和PET的组合。
This is the first multimodal study of cerebral tissue metabolism and perfusion post-hypoxic-ischaemic (HI) brain injury using broadband near-infrared spectroscopy (bNIRS), diffuse correlation spectroscopy (DCS), positron emission tomography (PET) and magnetic resonance spectroscopy (MRS). In seven piglet preclinical models of neonatal HI, we measured cerebral tissue saturation (StO2), cerebral blood flow (CBF), cerebral oxygen metabolism (CMRO2), changes in the mitochondrial oxidation state of cytochrome c oxidase (oxCCO), cerebral glucose metabolism (CMRglc) and tissue biochemistry (Lac+Thr/tNAA). At baseline, the parameters measured in the piglets that experience HI (not controls) were 64 ± 6% StO2, 35 ± 11 ml/100 g/min CBF and 2.0 ± 0.4 μmol/100 g/min CMRO2. After HI, the parameters measured were 68 ± 6% StO2, 35 ± 6 ml/100 g/min CBF, 1.3 ± 0.1 μmol/100 g/min CMRO2, 0.4 ± 0.2 Lac+Thr/tNAA and 9.5 ± 2.0 CMRglc. This study demonstrates the capacity of a multimodal set-up to interrogate the pathophysiology of HIE using a combination of optical methods, MRS, and PET.