Dysfunction of CD27+IgD+B cells correlates with aggravated systemic lupus erythematosus
Dysfunction of CD27+IgD+B cells correlates with aggravated systemic lupus erythematosus
复制标题
CD27 IgD B 细胞功能障碍与系统性红斑狼疮加重相关
DOI：
10.1007/s10067-022-06051-z
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发表时间：
2022-01-15
影响因子：
3.4
通讯作者：
Li，Ke
中科院分区：
文献类型：
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作者：
Zhang，Wei;Wang，Yong-Fu;Li，Ke
目的：系统性红斑狼疮（SLE）患者存在明显的细胞凋亡信号通路紊乱。天然IgM（nIgM）在清除凋亡细胞和防止它们触发有害的自身免疫中是重要的。B-1和先天性样B（ILB）细胞是主要的nIgM产生者。人CD 27 +IgD+B细胞（非转换记忆B细胞）被认为是ILB。方法：收集50例SLE患者和50例健康对照者的外周血，并对12例SLE患者进行随访研究。用流式细胞术分析每个群体中CD 27 +IgD+B细胞的量。ELISA POT法检测CD 27 +IgD+B细胞IgM水平，qRT-PCR法检测IL-10水平。数据分析采用SPSS17.0（SPSS，USA）。结果：SLE患者外周血CD 27 +IgD+B细胞数量明显低于健康对照组（P < 0.01）。CD 27 +IgD+B细胞数量与WBC呈正相关（r = 0.337，P = 0.017），血小板计数（r = 0.396，P = 0.004），血清C3水平与血清肌酐水平呈负相关（r = 0.415，P = 0.003）SLEDAI（r =-0.724，P = 0.000）和抗dsDNA（r =-0.477，P = 0.000）。活动期SLE患者CD 27 +IgD+B的IgM和IL-10水平较健康对照组明显降低（P < 0.001）。结论：SLE患者外周血中CD 27 + IgD + B细胞数量较正常人明显减少，CD 27 + IgD + B细胞与SLE患者的临床及免疫学特征相关。活动期SLE患者CD 27 +IgD+B细胞功能受损，产生IgM和IL-10。SLE患者治疗后病情缓解，CD 27 +IgD+B细胞数量恢复正常。关键点·SLE患者的CD 27 +IgD+B细胞数量显著低于健康对照。·SLE患者中CD 27 +IgD+B细胞在产生天然抗体样IgM和IL-10方面功能受损。·CD 27 +IgD+B细胞数量与SLE的临床和免疫学特征相关。
Objective:The apoptotic signaling pathway is obviously disordered in systemic lupus erythematosus (SLE). Natural IgM (nIgM) is important in clearing apoptotic cells and preventing them from triggering deleterious autoimmunity. B-1 and innate-like B (ILBs) cells are the main nIgM producers. Human CD27+IgD+B cells (un-switched memory B cells) are considered ILBs. However, their functional properties in SLE remain undefined.Methods:Peripheral blood sample of 50 SLE patients and 50 healthy controls were collected, and twelve SLE patients were assessed in a follow-up study. The amount of CD27+IgD+B cell in each population was analyzed by flow cytometry. The IgM and IL-10 levels of CD27+IgD+B cell were assessed by ELISPOT and qRT-PCR, respectively. SPSS 17.0 (SPSS, USA) was employed for data analysis. P < 0.05 indicated statistical significance.Result:The amounts of CD27+IgD+B cell were significantly decreased in SLE patients than healthy control (P < 0.01). CD27+IgD+B cell amounts were positively correlated with WBC (r = 0.337, P = 0.017), platelet count (r = 0.396, P = 0.004), and serum C3 levels (r = 0.415, P = 0.003) and negatively correlated with serum creatinine levels (r = - 0.285, P = 0.045), SLEDAI(r = - 0.724, P = 0.000), and anti-dsDNA(r = - 0.477, P = 0.000). The IgM and IL-10 levels of CD27+IgD+B in active SLE were decreased than healthy control (P < 0.001). Moreover, CD27+IgD+B cells are increased in SLE cases after treatment than before treatment (P < 0.001).Conclusion:The amounts of CD27+IgD+B cell were significantly decreased in SLE patients compared with the healthy population, and CD27+IgD+B cell was verified to be correlated with clinical and immunological features in SLE patients. CD27+IgD+B cells had impaired function associated with IgM and IL-10 production in active SLE. Moreover, the amounts of CD27+IgD+B cells were recovered to the normal level in SLE cases with treatment-related disease remission. Key Points • CD27+IgD+B cell amounts are significantly decreased in SLE patients than healthy control. • CD27+IgD+B cells are functionally impaired in producing natural antibody-like IgM and IL-10 in SLE patients. • CD27+IgD+B cell amounts are correlated with clinical and immunological features in SLE.