Microglia promote the proliferation of neural precursor cells by secreting osteopontin.
Microglia promote the proliferation of neural precursor cells by secreting osteopontin.
复制标题
DOI：
10.1016/j.bbrc.2019.04.076
复制
发表时间：
2019-06
影响因子：
3.1
通讯作者：
Miwako Yamamiya;S. Tanabe;R. Muramatsu
中科院分区：
文献类型：
--
作者：
Miwako Yamamiya;S. Tanabe;R. Muramatsu
小胶质细胞是中枢神经系统的免疫细胞，通过与神经前体细胞（npc）相互作用，在大脑发育中起着至关重要的作用。有报道称，小胶质细胞通过吞噬、诱导细胞凋亡和促进细胞增殖来调节NPC的数量。脑室下区周围的小胶质细胞在大脑发育过程中表达骨桥蛋白。本研究研究了小胶质细胞在体外NPCs增殖中的作用，并确定了对NPCs增殖至关重要的OPN受体。在OPN中和抗体存在的情况下，与npc共培养的小胶质细胞可抑制OPN并减少小胶质细胞诱导的npc增殖。npc表达整合素αvβ3，已被鉴定为OPN受体。整合素αvβ3抑制剂西伦吉肽也能抑制小胶质细胞诱导的NPCs增殖。提示小胶质细胞通过opn -整合素αvβ3信号通路促进NPCs的增殖。
Microglia are central nervous system-resident immune cells that play a crucial role in brain development by interacting with neural precursor cells (NPCs). It has been reported that microglia regulate the number of NPC by phagocytosis, inducing apoptosis, and promoting proliferation. Microglia surrounding the subventricular zone express osteopontin (OPN) during brain development. The present study investigated the role of microglia in proliferation of NPCs in vitro, and identified the OPN receptor critical for proliferation of NPCs. Microglia co-cultured with NPCs in the presence of an OPN-neutralizing antibody resulted in OPN inhibition and reduced microglia-induced proliferation of NPCs. NPCs express integrin αvβ3, which has been identified as an OPN receptor. Cilengitide, an inhibitor of integrin αvβ3, also inhibited microglia-induced proliferation of NPCs. These results suggest that microglia promote the proliferation of NPCs via OPN-integrin αvβ3 signaling.