Retinoids enhance cisplatin-based chemoradiation in cervical cancer cells in vitro.
Retinoids enhance cisplatin-based chemoradiation in cervical cancer cells in vitro.
复制标题
类视黄醇在体外增强宫颈癌细胞中基于顺铂的放化疗。
DOI：
10.1006/gyno.2002.6590
复制
发表时间：
2002
影响因子：
4.7
通讯作者：
Benbrook,DorisM
中科院分区：
文献类型：
--
作者：
ScribnerJr,DennisR;Benbrook,DorisM
本研究的目的是确定受体依赖性和受体非依赖性维甲酸是否对宫颈癌细胞对临床相关剂量的同时放射和顺铂敏感。方法在SiHa宫颈癌细胞培养物上进行克隆形成试验，用5 μM 9-顺式维甲酸（RA）或3 μM 4-顺式维甲酸（RA）处理。结果9-cis-RA和4-HPR均未显著降低单纯放疗或单纯顺铂治疗的生存率（t检验：P < 0.05），但两者均显著降低同时接受放化疗的培养物的存活率（t检验：9-cis-RA P = 0.045; 4-HPR P = 0.027）。
ObjectiveThe aim of this study was to determine whether receptor-dependent and receptor-independent retinoids sensitize cervical cancer cells to clinically relevant doses of concurrent radiation and cisplatin.MethodsThe clonogenic assay was performed on SiHa cervical carcinoma cultures treated with 5 μM 9-cis-retinoic acid (RA) or 3 μM 4-HPR for 3 days prior to and following concurrent treatment with 3 μM cisplatin and 2 Gy of Co60radiation.ResultsNeither 9-cis-RA nor 4-HPR significantly decreased survival for radiation only or cisplatin only (t test: P < 0.05), but both significantly decreased survival of cultures receiving concurrent chemoradiation (t test: 9-cis-RA P = 0.045; 4-HPR P = 0.027).ConclusionsBoth receptor-dependent and receptor-independent retinoids enhance concurrent chemoradiation effects in vitro.