Decreased Expression of circ_0000160 in Breast Cancer With Axillary Lymph Node Metastasis.
Decreased Expression of circ_0000160 in Breast Cancer With Axillary Lymph Node Metastasis.
复制标题
乳腺癌腋窝淋巴结转移中 circ_0000160 表达降低
DOI：
10.3389/fmolb.2021.690826
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发表时间：
2021
影响因子：
5
通讯作者：
Su P
中科院分区：
文献类型：
--
作者：
Wang YW;Chen X;Tian Y;Liu L;Su P
背景：环状RNA（CircRNA）在人类肿瘤的发生、发展中起重要作用。新出现的证据表明，circRNA有可能成为癌症诊断和预后的有前途的生物标志物。然而，circRNA在乳腺癌腋窝淋巴结转移（ALNM）中的作用仍有待确定。 研究方法：利用转录组测序技术筛选3例ALNM和3例非ALNM乳腺癌组织中差异表达的circRNA。通过定量实时PCR进一步验证差异表达的circRNA。此外，进行受试者工作特征（ROC）曲线分析以计算circRNA的值来区分有ALNM和无ALNM的乳腺癌组织。为了探索circRNA的潜在机制，基于CircInteractome、circBank和mirDIP在线软件构建了circRNA-miRNA-mRNA网络。 结果如下：通过转录组测序，共鉴定出31种差异表达的circRNA;其中，与未进行ALNM的乳腺癌患者相比，ALNM患者中有21种表达上调，10种表达下调。与没有ALNM的乳腺癌组织相比，Circ_0000160被证实在有ALNM的乳腺癌组织中下调。ROC曲线显示，circ_0000160能够区分有ALNM和无ALNM的乳腺癌组织，曲线下面积为0.7435。此外，生物信息学分析显示，预测的mRNAs circ_0000160可能与淋巴结转移有关。circ_0000160的预测mRNA可能参与许多癌症相关途径。 结论：circ_0000160在有腋淋巴结转移的乳腺癌中表达降低。Circ_0000160可能具有区分有腋窝淋巴结转移的乳腺癌和无腋窝淋巴结转移的乳腺癌的潜力。
Background: Circular RNAs (circRNAs) have been shown to play important roles in the development and progression of human cancers. Emerging evidence shows that circRNAs have the potential to be promising biomarkers for cancer diagnosis and prognosis. However, the roles of circRNAs in breast cancer axillary lymph node metastasis (ALNM) remain to be determined. Methods: Transcriptome sequencing was utilized to screen the differentially expressed circRNAs in three breast cancer tissues with ALNM and three without ALNM. Differentially expressed circRNAs were further verified by quantitative real-time PCR. Moreover, receiver operating characteristic (ROC) curve analysis was performed to calculate the value of circRNAs to distinguish breast cancer tissues with ALNM and those without ALNM. To explore the potential mechanism of the circRNAs, a circRNA–miRNA–mRNA network was constructed based on the CircInteractome, circBank, and mirDIP online software. Results: In total, 31 differentially expressed circRNAs were identified by transcriptome sequencing; among them, 21 were upregulated and 10 were downregulated in breast cancer with ALNM compared to those without ALNM. Circ_0000160 was validated to be downregulated in breast cancer tissues with ALNM compared with those without ALNM. The ROC curve showed the ability of circ_0000160 to distinguish breast cancer tissues with ALNM and those without ALNM, with an area under the curve of 0.7435. Furthermore, bioinformatics analysis revealed that the predicted mRNAs for circ_0000160 may be related to lymph node metastasis. The predicted mRNAs for circ_0000160 may be involved in many cancer-related pathways. Conclusion: A decreased expression of circ_0000160 was found in breast cancer with axillary lymph node metastasis. Circ_0000160 may have the potential to distinguish breast cancer with axillary lymph node metastasis from those without axillary lymph node metastasis.