Bimodal clock gene expression in mouse suprachiasmatic nucleus and peripheral tissues under a 7-hour light and 5-hour dark schedule
Bimodal clock gene expression in mouse suprachiasmatic nucleus and peripheral tissues under a 7-hour light and 5-hour dark schedule
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DOI：
10.1177/0748730406295435
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发表时间：
2007-02-01
影响因子：
3.5
通讯作者：
Ebihara, Shizufumi
中科院分区：
文献类型：
--
作者：
Watanabe, Tsuyoshi;Naito, Emiko;Ebihara, Shizufumi
使用mPer 1：：luc实时监测技术，作者观察到SCN两侧mPer 1生物发光的双峰模式，同时在人工光：暗：光：暗（LDLD）7：5：7：5条件下保持SCN左右两侧之间的同步。原位杂交分析mPer 1和mBmal 1 mRNA在SCN中的分布发现，在LDLD的I期（晨期; M），mPer 1在SCN的腹外侧样亚区（VL样亚区）的表达水平高于背内侧样亚区（DM样亚区），而在另一个I期（晚期; E），这种区域分布模式则相反。与此相反，在M期，mBmal 1水平在DM样亚部中高于VL样亚部，并且这种分布在E期发生变化。在DM样和VL样SCN中，编码SCN输出分子（被认为传递昼夜运动节律）的前动力蛋白2（PK 2）mRNA均减少，并且与LDLD条件下的活性无明显相关性。mPer 1和mPer 2在肝脏中的表达明显呈双峰，而其他时钟基因的表达与LDLD条件不同步。这些结果可能会提供重要的见解分裂或双峰节律，反过来可能有助于理解的能力，以测量哺乳动物的季节性日长的机制。
Using the mPer1::luc real-time monitoring technique, the authors observed the bimodal patterns of mPer1 bioluminescence on each side of the SCN, in parallel with maintaining synchronization between the left and right sides of the SCN under an artificial light:dark:light:dark (LDLD) 7:5:7:5 condition. In situ hybridization analysis of mPer1 and mBmal1 mRNA distribution in the SCN showed that in I photophase (morning photophase; M) of LDLD, the mPer1 level in the ventrolateral-like (VL-like) subdivision of the SCN was higher than that in the dorsomedial-like (DM-like) subdivision, and this regional distribution pattern was reversed in another photophase (evening photophase; E). In contrast, the mBmal1 level was higher in the DM-like subdivision than in the VL-like subdivision in the M phase, and this distribution changed in the E phase. The prokineticin 2 (PK2) mRNA that encodes an SCN output molecule that is thought to transmit the circadian locomotor rhythms was reduced in both the DM-like and VL-like SCN and did not clearly correlate with the activity under the LDLD condition. The expression of mPer1 and mPer2 in the liver was clearly bimodal, whereas the expressions of other clock genes were not synchronized to the LDLD condition. These results may provide important insights into the mechanism underlying the splitting or bimodal rhythms that may in turn facilitate the understanding of the ability to measure the seasonal day length in mammals.