Functional characterization of a peroxisome proliferator response-element located in the intron 3 of rat peroxisomal thiolase B gene
Functional characterization of a peroxisome proliferator response-element located in the intron 3 of rat peroxisomal thiolase B gene
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DOI：
10.1016/j.bbrc.2003.09.185
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发表时间：
2003-11-07
影响因子：
3.1
通讯作者：
Nicolas-Francés, V
中科院分区：
文献类型：
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作者：
Hansmannel, F;Clémencet, MC;Nicolas-Francés, V
大鼠过氧化物酶体3-酮酰辅酶A硫解酶基因B的表达是由过氧化物酶体增殖物诱导的。虽然在该基因的启动子区域有一个类似PPAR结合位点的序列元件，但我们先前发现该元件能被肝细胞核因子-4激活，但不能与PPARpha结合。在此，我们描述了一个位于内含子3(+1422/+1434)的新的过氧化物酶体增殖物反应元件，它在体外与PPARpha/维甲酸X受体的异源二聚体结合，并在转染实验中被PPARpha诱导。我们提出了一个涉及启动子和内含子3中的那些元件的大鼠硫解酶B基因调控模型。(C)2003 Elsevier Inc.保留所有权利。
Expression of the rat peroxisomal 3-ketoacyl-CoA thiolase gene B is induced by peroxisome proliferators. Although a sequence element like a peroxisome proliferator-activated receptor (PPAR)-binding site is located in the promoter region of this gene, we previously found that this element is competent for the activation by hepatocyte nuclear factor-4, but not functional with PPARalpha. We describe here a new peroxisome proliferator-response element located in the intron 3 (+1422/+1434) that binds in vitro the PPARalpha/retinoid X receptor a heterodimer and confers the induction by PPARalpha in transfection assays. We propose a model of regulation of the rat thiolase B gene involving those elements in the promoter and intron 3. (C) 2003 Elsevier Inc. All rights reserved.