Vasopressin RNA in the neural lobe of the pituitary: dramatic accumulation in response to salt loading.
Vasopressin RNA in the neural lobe of the pituitary: dramatic accumulation in response to salt loading.
复制标题
垂体神经叶中的加压素 RNA：响应盐负荷而急剧积累。
DOI：
10.1073/pnas.86.22.9002
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
D. Carter
中科院分区：
文献类型：
--
作者：
D. Murphy;A. Levy;S. Lightman;D. Carter
加压素（VP）和催产素是由高度同源的连锁基因编码的前激素原编码的肽，这些基因在下丘脑神经元的离散群中表达。成熟激素从垂体的神经（后）叶释放到外周循环中，并且也参与前叶的调节。我们已经使用北方印迹和原位杂交的RNA在组织切片中描述的存在，解剖定位，和调节VP和催产素RNA在垂体本身。我们无法检测到垂体前叶和中叶的VP转录。相反，我们在神经叶中发现了低水平的VP RNA。此外，渗透刺激的2%（重量/体积）NaCl饮用饮食导致VP RNA在神经叶中的显着积累。我们认为，VP，局部合成的垂体细胞，可能有旁分泌的VP受体在神经叶的影响。
The peptides vasopressin (VP) and oxytocin are derived from preprohormone precursers encoded by highly homologous linked genes that are expressed in discrete groups of hypothalamic neurons. The mature hormones are released into the peripheral circulation from the neural (posterior) lobe of the pituitary and have also been implicated in the regulation of anterior lobe. We have used Northern blotting and in situ hybridization to RNA in tissue sections to describe the presence, anatomical localization, and regulation of VP and oxytocin RNAs in the pituitary gland itself. We were unable to detect VP transcripts in the anterior and intermediate lobes of the pituitary. Rather we found low levels of VP RNA in the neural lobe. Furthermore, the osmotic stimulation of a 2% (wt/vol) NaCl drinking diet resulted in a marked accumulation of VP RNA in the neural lobe. We suggest that VP, locally synthesized in pituicytes, may have paracrine effects on VP receptors in the neural lobe.