Silver nanoparticles induce developmental toxicity via oxidative stress and mitochondrial dysfunction in zebrafish (Danio rerio)
Silver nanoparticles induce developmental toxicity via oxidative stress and mitochondrial dysfunction in zebrafish (Danio rerio)
复制标题
银纳米粒子通过氧化应激和线粒体功能障碍诱导斑马鱼（斑马鱼）的发育毒性
DOI：
10.1016/j.ecoenv.2022.113993
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发表时间：
2022-08-19
影响因子：
6.8
通讯作者：
Yang, Xiaojun
中科院分区：
文献类型：
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作者：
Lu, Chunjiao;Lv, Yuhang;Yang, Xiaojun
纳米银颗粒（AgNPs）广泛应用于工业、农业和医学领域，可能对人类健康和水生环境产生不利影响。在这里，我们研究了急性暴露于 AgNP 的斑马鱼胚胎的发育毒性。我们的结果表明，4 hpf 斑马鱼胚胎暴露于不同浓度的 AgNP 72 小时后出现发育缺陷。此外，对 AgNP 处理后的斑马鱼胚胎进行 RNA 测序分析。进一步的基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，AgNPs处理组的差异表达基因（DEG）在DNA复制起始、氧化还原酶活性、DNA复制、细胞衰老和氧化磷酸化信号通路方面富集。值得注意的是，我们还发现AgNPs暴露可导致活性氧（ROS）和丙二醛（MDA）的积累，超过氧化物歧化酶（SOD）、过氧化氢酶（CAT）和线粒体复合物I-V活性的抑制，以及SOD、CAT和线粒体复合物I-IV链相关基因的表达下调。此外，斑马鱼暴露于AgNPs后，线粒体介导的凋亡信号通路相关基因（例如bax、bcl2、caspase-3和caspase-9）的表达受到显着调节。因此，这些研究结果表明，AgNPs 暴露可能引起氧化应激，诱导线粒体功能障碍，并最终导致发育毒性。
Sliver nanoparticles (AgNPs) are widely used in industry, agriculture, and medicine, potentially resulting in adverse effects on human health and aquatic environments. Here, we investigated the developmental toxicity of zebrafish embryos with acute exposure to AgNPs. Our results demonstrated developmental defects in 4 hpf zebrafish embryos after exposure to different concentrations of AgNPs for 72 h. In addition, RNA-seq profiling of zebrafish embryos after AgNPs treatment. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the differentially expressed genes (DEGs) were enriched in DNA replication initiation, oxidoreductase activity, DNA replication, cellular senescence, and oxidative phos-phorylation signaling pathways in the AgNPs-treated group. Notably, we also found that AgNPs exposure could result in the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), the inhibition of su-peroxide dismutase (SOD), catalase (CAT), and mitochondrial complex I-V activities, and the downregulated expression of SOD, CAT, and mitochondrial complex I-IV chain-related genes. Moreover, the expression of mitochondrion-mediated apoptosis signaling pathway-related genes, such as bax, bcl2, caspase-3, and caspase-9, was significantly regulated after AgNPs exposure in zebrafish. Therefore, these findings demonstrated that AgNPs exposure could cause oxidative stress, induce mitochondrial dysfunction, and ultimately lead to developmental toxicity.