Killer cell immunoglobulin-like receptor gene diversity in a Southern Brazilian population from the state of Parana
Killer cell immunoglobulin-like receptor gene diversity in a Southern Brazilian population from the state of Parana
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DOI：
10.1016/j.humimm.2008.09.002
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发表时间：
2008-12-01
期刊：
影响因子：
2.7
通讯作者：
Laguila Visentainer, Jeane Eliete
中科院分区：
文献类型：
--
作者：
Chagas Rudnick, Cristiane Conceicao;Alessio Franceschi, Danilo Santana;Laguila Visentainer, Jeane Eliete
杀伤细胞免疫球蛋白样受体（KIR）是一种由多态性基因编码并结合人类白细胞抗原（HLA） 1类分子的受体。本研究的目的是调查巴西南部巴拉那州人群中KIR基因和抑制性KIR/HLA对的分布，并将结果与其他人群的结果进行比较。利用反序列特异性寡核苷酸Luminex （One Lambda, Inc., Canoga Park， CA）对289名非亲疏个体的16个KIR基因和HLA 1类等位基因进行基因分型。这个巴西人群在KIR基因的频率方面与高加索人群有几个相似之处。共有38种基因型，其中纯合单倍型A最为常见（33.2%）。因此，有可能确定两个新的基因型。大多数个体表现出至少一个抑制KIR/HLA对。2对最常见（40.4%），其次是3对（38.2%）、1对（14.6%）和4对（6.4%）。以KlR2DL2/3 + HLA-C1对最常见（79.9%），KIR3DL2 + HLA-A3/11对最不常见（25.0%）。本研究证明了Parana人群中KIR基因的多样性，以及具有种族混合的高加索人的特征模式，从而定义了两个新的基因型，并鉴定了一个没有抑制KIR/HLA对的个体。(C) 2008年美国组织相容性与免疫遗传学学会。Elsevier Inc.出版。版权所有。
Killer cell immunoglobulin-like receptors (KIR) are encoded by polymorphic genes and have as binding human leukocyte antigen (HLA) class 1 molecules. The aim of this study was to investigate the distribution of KIR genes and inhibitory KIR/HLA pairs in a population from Southern Brazil, in the state of Parana, and to compare the results with results from other populations. The genotyping of 16 KIR genes and HLA class 1 alleles of 289 unrelated individuals was accomplished by reverse sequence-specific oligonucleotide Luminex (One Lambda, Inc., Canoga Park, CA). This Brazilian population demonstrated several similarities to Caucasian populations with regard to the frequency of KIR genes. Thirty-eight genotypes were defined in which the most frequent was the homozygous haplotype A (33.2%). Therefore, it was possible to define two new genotypes. Most of the individuals demonstrated at least one inhibitory KIR/HLA pair. Two pairs were the most frequent (40.4%), followed by three pairs (38.2%), one pair (14.6%), and four pairs (6.4%). The KlR2DL2/3 + HLA-C1 pair was the most frequent (79.9%) and the least frequent pair was KIR3DL2 + HLA-A3/11 (25.0%). This study demonstrated the diversity of KIR genes in a population of Parana, as well as the characteristic pattern of Caucasians with racial admixture, which enabled the definition of two new genotypes and the identification of one individual without the inhibitory KIR/HLA pair. (C) 2008 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.