A role of GUNs-Involved retrograde signaling in regulating Acetyl-CoA carboxylase 2 in Arabidopsis.
A role of GUNs-Involved retrograde signaling in regulating Acetyl-CoA carboxylase 2 in Arabidopsis.
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DOI：
10.1016/j.bbrc.2018.09.144
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发表时间：
2018-11
影响因子：
3.1
通讯作者：
Juan Wang;H. Xia;Shuzhen Zhao;L. Hou;Chuanzhi Zhao;Chang-Le Ma;Xingjun Wang;Pengcheng Li
中科院分区：
文献类型：
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作者：
Juan Wang;H. Xia;Shuzhen Zhao;L. Hou;Chuanzhi Zhao;Chang-Le Ma;Xingjun Wang;Pengcheng Li
在拟南芥（Arabidopsis sisthaliana）中，乙酰辅酶a羧化酶2 （Acetyl-CoA Carboxylase 2, ACC2）是一种核dna编码的质体靶向酶，可催化乙酰辅酶a转化为丙二酰辅酶a。当叶绿体翻译受损时，ACC2促进植物生长发育。然而，对调控acc2的上游信号知之甚少。本研究通过分析brz- insensitivity -pale green(bpg)2-2（一个由于BPG2功能丧失而导致叶绿体基因表达受损的浅绿色突变体）的转录组变化，我们发现facc2水平显著上调。通过遗传分析，我们进一步证明了基因组UNCOUPLED 1 （GUN1）或GUN5功能的缺失在一定程度上扰乱了bpg2-2突变体中facc2的上调，而ABA不敏感4 （ABI4）功能的缺失对acc2的表达没有明显影响。此外，当植物被林可霉素和大观霉素等质体翻译抑制剂处理时，acc2的转录水平也以gun依赖的方式显著增加。总之，我们的研究结果表明，gun参与的质体-核逆行通信在拟南芥acc2调控中发挥了作用。
InArabidopsisthaliana(Arabidopsis), Acetyl-CoA Carboxylase 2 (ACC2) is a nuclear DNA-encoded and plastid-targeted enzyme that catalyzes the conversion of acetyl-CoA to malonyl-CoA. ACC2 improves plant growth and development when chloroplast translation is impaired. However, little is known about the upstream signals that regulateACC2. Here, through analyzing the transcriptome changes inbrz-insensitive-pale green(bpg)2-2, a pale-green mutant with impaired chloroplast gene expression resulting from loss of the BPG2 function, we found that the level ofACC2was significantly up-regulated. Through performing genetic analysis, we further demonstrated that loss of the GENOMES UNCOUPLED 1 (GUN1) or GUN5 function partly perturbed the up-regulation ofACC2in thebpg2-2mutant, whereas ABA INSENSITIVE 4 (ABI4)-function-loss had no clear effect on theACC2expression. Furthermore, when plants were treated with plastid translation inhibitors, such as lincomycin and spectinomycin, theACC2transcriptional level was also markedly increased in a GUN-dependent manner. In conclusion, our results suggested that the GUN-involved plastid-to-nucleus retrograde communication played a role in regulatingACC2in Arabidopsis.