Cells of the mononuclear phagocytic system resorb implanted bone matrix: A histologic and ultrastructural study
Cells of the mononuclear phagocytic system resorb implanted bone matrix: A histologic and ultrastructural study
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单核吞噬系统细胞吸收植入骨基质：组织学和超微结构研究
DOI：
10.1007/bf02411290
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发表时间：
1982
影响因子：
4.2
通讯作者：
J. Glowacki
中科院分区：
文献类型：
--
作者：
M. Holtrop;K. Cox;J. Glowacki
将含矿物质的骨碎片植入大鼠颅骨缺损中，可启动骨基质的快速和可重复吸收。7天后，骨碎片周围和骨碎片之间形成致密组织，单核细胞和多核细胞。电子显微镜显示，这些细胞属于单核吞噬细胞系统;它们被鉴定为巨噬细胞、上皮样细胞、异物巨细胞和朗格汉斯细胞。除了共同的超微结构特征外，这些细胞沿其细胞膜具有电子致密的局灶性特化（沿着其细胞膜，外部有涂层），对应于质膜下线性密度。小的，未识别的细胞与电子致密的地面细胞质中经常看到接近更分化的细胞。骨碎片表面未发生粘连。类似Howship腔隙的凹痕是常见的;这些包含单核细胞以及多核细胞。一些表面磨损，胶原纤维暴露，但与这些表面贴壁的细胞没有破骨细胞中所见的皱褶边界。一些骨头碎片被打碎，细胞突起深入裂缝，将基质碎片分开。小基质颗粒被巨噬细胞吞噬，但不被上皮样细胞或巨细胞吞噬。看来，能够降解骨基质成分的酶是由单核吞噬细胞系统中分化程度更高的细胞分泌的。它们侵蚀骨表面的方式让人联想到骨细胞的骨吸收。它们也从骨碎片中进入小管，发挥作用，这是一个只有在死骨中才可能发生的过程。我们建议这些细胞与破骨细胞的可能关系。
SummaryImplantation of mineral-containing bone fragments into calvarial defects in rats initiates a rapid and reproducible resorption of the bone matrix. After 7 days, a dense tissue develops with mononucleated as well as multinucleated cells surrounding and between the bone fragments. Electron microscopy revealed that these cells belong to the mononuclear phagocytic system; they were identified as macrophages, epithelioid cells, foreign body giant cells, and Langerhans cells. In addition to the common ultrastructural characteristics, these cells had electron-dense, focal specializations along their cell membrane with a coating on the exterior, corresponding to subplasmalemmal linear densities. Small, unidentified cells with electron-dense ground cytoplasm were often seen in close proximity to more differentiated cells. No halisteresis had occurred on the surfaces of the bone fragments. Indentations resembling Howship's lacunae were frequent; these contained mononucleated as well as multinucleated cells. Some surfaces were frayed and collagen fibers were exposed, but the cells apposed to these surfaces did not have ruffled borders as are seen in osteoclasts. Some bone fragments were broken up and cell processes had penetrated deep into the cracks, separating pieces of matrix. Small matrix particles were phagocytosed by macrophages, but not by epithelioid cells or giant cells. It appears that enzymes capable of degrading bone matrix components were secreted by the more differentiated cells of the mononuclear phagocytic system. They eroded the bone surface in a way reminiscent of osteoclastic bone resorption. They also entered the canaliculi to act from within the bone fragment, a process possible only in dead bone. We suggest a possible relationship of these cells with osteoclasts.