Two simple and rugged designs for creating microfluidic sheath flow
Two simple and rugged designs for creating microfluidic sheath flow
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DOI：
10.1039/b719381e
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Ligler, Frances S.
中科院分区：
文献类型：
--
作者：
Howell, Peter B., Jr.;Golden, Joel P.;Ligler, Frances S.
提出并成功演示了一种能够进行二维流体动力聚焦的简单设计。过去，大多数微流体鞘流系统通常仅将样品溶液限制在侧面，使样品流的顶部和底部与通道的底部和顶部接触。虽然构建相对简单，但这些设计增加了样品成分吸附到通道顶部和底部的风险。一些设计已成功地完全覆盖样本流，但这些通常需要多个护套输入和多个对准步骤。在此介绍的设计中，只需使用一个护套输入即可完成完整护套，从而无需仔细平衡两个或多个护套入口的流量。该设计可以使用当前的微加工技术轻松制造。此外，样品和鞘液可以随后分离以重新捕获样品液或重新使用鞘液。使用软光刻在聚（二甲基硅氧烷）（PDMS）和使用微铣削和激光烧蚀的聚（甲基丙烯酸甲酯）（PMMA）中演示了设计。
A simple design capable of 2-dimensional hydrodynamic focusing is proposed and successfully demonstrated. In the past, most microfluidic sheath flow systems have often only confined the sample solution on the sides, leaving the top and bottom of the sample stream in contact with the floor and ceiling of the channel. While relatively simple to build, these designs increase the risk of adsorption of sample components to the top and bottom of the channel. A few designs have been successful in completely sheathing the sample stream, but these typically require multiple sheath inputs and several alignment steps. In the designs presented here, full sheathing is accomplished using as few as one sheath input, which eliminates the need to carefully balance the flow of two or more sheath inlets. The design is easily manufactured using current microfabrication techniques. Furthermore, the sample and sheath fluid can be subsequently separated for recapture of the sample fluid or re-use of the sheath fluid. Designs were demonstrated in poly(dimethylsiloxane) (PDMS) using soft lithography and poly(methyl methacrylate) (PMMA) using micromilling and laser ablation.