Unusual gene order and organization of the sea urchin Hox cluster
Unusual gene order and organization of the sea urchin Hox cluster
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DOI：
10.1002/jez.b.21070
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发表时间：
2006-01-15
影响因子：
2.2
通讯作者：
Hood, L
中科院分区：
文献类型：
--
作者：
Cameron, RA;Rowen, L;Hood, L
虽然高度一致的基因顺序和轴向共线模式的表达似乎是脊椎动物盒基因簇的一个特点，这种模式可能是不太好的保守在其余的两侧。我们报告的第一个后口实例的一个完整的盒簇与一个独特的基因顺序，其中的parabolic组不表示在一个连续的方式。来自海胆（Strongylocentrotus purpuratus）基因组的BAC克隆的完成序列揭示了基因顺序，其中在簇中，前基因（Hox 1、Hox 2和Hox 3）最靠近后基因，使得最3'基因是Hox 5。(The基因顺序为5-Hox 1、2、3、11/13 c、11/13 b、11/13 a、9/10、8、7、6、5- 3 '。通过限制性酶切图谱证据和BAC末端支架分析证实了完成的序列结果。与一个假定的祖先后口类Hox基因簇的比较表明，导致海胆基因顺序的重排是许多和复杂的。
While the highly consistent gene order and axial colinear patterns of expression seem to be a feature of vertebrate box gene clusters, this pattern may be less well conserved across the rest of the bilaterians. We report the first deuterostome instance of an intact box cluster with a unique gene order where the paralog groups are not expressed in a sequential manner. The finished sequence from BAC clones from the genome of the sea urchin, Strongylocentrotus purpuratus, reveals a gene order wherein the anterior genes (Hox1, Hox2 and Hox3) lie nearest the posterior genes in the cluster such that the most 3' gene is Hox5. (The gene order is 5-Hox1, 2, 3, 11/13c, 11/13b, 11/13a, 9/10, 8, 7, 6, 5-3'.) The finished sequence result is corroborated by restriction mapping evidence and BAC-end scaffold analyses. Comparisons with a putative ancestral deuterostome Hox gene cluster suggest that the rearrangements leading to the sea urchin gene order were many and complex.