Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis.
Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis.
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DOI：
10.1083/jcb.200309082
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发表时间：
2004-02-16
期刊：
影响因子：
--
通讯作者：
Youle RJ
中科院分区：
文献类型：
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作者：
Karbowski M;Arnoult D;Chen H;Chan DC;Smith CL;Youle RJ
细胞器融合和裂变的动态平衡调节线粒体形态。在细胞凋亡过程中，这种平衡被改变，导致线粒体广泛断裂。在这里，我们描述了一种新的线粒体动力学分析方法，该方法基于表达线粒体基质靶向光激活绿色荧光蛋白的细胞共聚焦成像，可以检测和定量活细胞中的细胞器融合。使用这种方法，我们可视化和量化了健康细胞和凋亡细胞的线粒体融合率。在细胞凋亡过程中，线粒体融合不依赖于caspase的激活而被阻断。线粒体融合阻滞发生的时间范围与Bax在线粒体和线粒体外膜上的聚结时间相同，这可能是细胞凋亡过程中Bax/Bak活化的结果。
A dynamic balance of organelle fusion and fission regulates mitochondrial morphology. During apoptosis this balance is altered, leading to an extensive fragmentation of the mitochondria. Here, we describe a novel assay of mitochondrial dynamics based on confocal imaging of cells expressing a mitochondrial matrix–targeted photoactivable green fluorescent protein that enables detection and quantification of organelle fusion in living cells. Using this assay, we visualize and quantitate mitochondrial fusion rates in healthy and apoptotic cells. During apoptosis, mitochondrial fusion is blocked independently of caspase activation. The block in mitochondrial fusion occurs within the same time range as Bax coalescence on the mitochondria and outer mitochondrial membrane permeabilization, and it may be a consequence of Bax/Bak activation during apoptosis.