Dual Effect of Nonionic Surfactants on Improving the Enzymatic Hydrolysis of Lignocellulose
Dual Effect of Nonionic Surfactants on Improving the Enzymatic Hydrolysis of Lignocellulose
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非离子表面活性剂改善木质纤维素酶解的双重作用
DOI：
10.1021/acs.energyfuels.8b00225
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发表时间：
2018
期刊：
影响因子：
5.3
通讯作者：
Yuan Zhenhong
中科院分区：
文献类型：
--
作者：
Wang Wen;Zhuang Xinshu;Tan Xuesong;Wang Qiong;Chen Xiaoyan;Yu Qiang;Qi Wei;Wang Zhongming;Yuan Zhenhong
非离子表面活性剂提高纤维素转化率的机理目前仍存在争议。保护酶的稳定性和防止纤维素酶对木质素的非生产性吸附是非离子表面活性剂促进纤维素水解的主要因素。SDS-PAGE检测表明，无论聚山梨醇酯（吐温）存在与否，酶在酶解过程中都不会发生聚集。吐温具有不同的保持甚至提高酶活性的能力，但这些能力与提高处理甘蔗渣（SCB）的酶解效果关系不大。吐温能增加纤维素酶对木质素和SCB样品的吸附，这与目前认为非离子表面活性剂会阻碍纤维素酶对木质素的吸附的观点不同。讨论认为，非离子表面活性剂对纤维素酶与底物的接触起润滑作用。
The mechanism of nonionic surfactants improving the cellulose conversion is still controversial. To protect enzyme stability and prevent unproductive adsorption of cellulase to lignin, which have been thought of as the chief factors for the improvement of nonionic surfactants on cellulolytic hydrolysis, were evaluated in this work. SDS-PAGE detection showed that the enzyme could not aggregate in the enzymatic hydrolysis process whether the polysorbates (Tweens) were present or not. Tweens had different capabilities to retain and even improve enzymes’ activities, but these capabilities had little relation to enhancing the enzymatic hydrolysis of treated sugar cane bagasse (SCB). Tweens could increase the adsorption of cellulase to lignins and SCB samples, which was different from the current viewpoint that nonionic surfactants could impede the adsorption of cellulase to lignin. After discussion, it was proposed that the nonionic surfactants initially lubricated the access of cellulase to cellulose and subs...