A Calcium-Cross-Linked Hydrogel Based on Alginate-Modified Atelocollagen Functions as a Scaffold Material
A Calcium-Cross-Linked Hydrogel Based on Alginate-Modified Atelocollagen Functions as a Scaffold Material
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DOI：
10.1163/092050611x555678
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发表时间：
2012-01
期刊：
影响因子：
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通讯作者：
W. Kamimura;Rieko Hattori;H. Koyama;T. Miyata;T. Takato
中科院分区：
文献类型：
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作者：
W. Kamimura;Rieko Hattori;H. Koyama;T. Miyata;T. Takato
我们开发了一种由海藻酸盐和胶原蛋白（AtCol）共价键结构组成的支架材料。钙离子的加入使材料形成水凝胶（海藻酸盐修饰的AtCol凝胶）。海藻酸盐改性AtCol凝胶的制备条件可由海藻酸盐的投料比和活化剂控制。在培养基中的时间稳定性测试表明，藻酸盐与AtCol之间的共价键可能有助于藻酸盐修饰的AtCol凝胶的结构稳定性。内皮细胞培养表明，海藻酸修饰的AtCol凝胶与天然胶原的粘附性相似。胶原酶消化表明，海藻酸修饰的AtCol凝胶具有相当大的保留碱性成纤维细胞生长因子的能力。此外，通过内皮细胞的体外实验，检测到活性细胞向海藻酸盐修饰的AtCol迁移。这些发现表明，这种凝胶材料可以作为诱导血管生长的支架。
We have developed a scaffold material consisting of a covalently-bonded structure of alginate and atelocollagen (AtCol). Addition of calcium ions caused the material to form a hydrogel (alginate-modified AtCol gel). The condition of the alginate-modified AtCol gel could be controlled by the feed ratio of alginate and the activating reagent. Measurement of temporal stability in culture medium suggested that covalent bonding between alginate and AtCol might contribute to the structural stability of the alginate-modified AtCol gel. Culture with endothelial cells indicated that cell adhesiveness on the alginate-modified AtCol gel was similar to that on native collagen. Collagenase digestion revealed that the alginate-modified AtCol gel had considerable ability to retain basic fibroblast growth factor. Additionally, active cell migration into alginate-modified AtCol was detected by in vitro assay using endothelial cells. These findings indicate that this gel material can be expected to function as a scaffold for inducing vascular in-growth.