Twenty microsatellite markers for the endangered Vatica mangachapoi (Dipterocarpaceae).
Twenty microsatellite markers for the endangered Vatica mangachapoi (Dipterocarpaceae).
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DOI：
10.3732/apps.1600134
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发表时间：
2017-02
影响因子：
3.6
通讯作者：
Zeng J
中科院分区：
文献类型：
--
作者：
Guo JJ;Shang SB;Wang CS;Zhao ZG;Zeng J
利用微卫星标记技术对原产于东南亚和中国南方的濒危植物青皮进行了研究。利用高通量测序技术，从芒刺葡萄中鉴定出20个微卫星标记，其中多态性标记12个。在每个标记的多态性进行了评估，使用87个人从三个自然群体。每个多态位点的等位基因数在6 ~ 15个之间，观察杂合度在0.000 ~ 0.926之间，期望杂合度在0.177 ~ 0.864之间。这些标记已成功转移到濒危物种广西葡萄中。这些标记可用于研究芒刺葡萄和广西葡萄的遗传多样性和基因流。
Microsatellite markers were developed for Vatica mangachapoi (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China. Twenty microsatellite markers, including 12 polymorphic markers, were identified from V. mangachapoi using high-throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0.000 to 0.926 and from 0.177 to 0.864, respectively. These markers were transferred successfully to the endangered species V. guangxiensis. These markers may be used to investigate the genetic diversity and gene flow of V. mangachapoi and V. guangxiensis.