Precise electrokinetic position and three-dimensional orientation control of a nanowire bioprobe in solution
Precise electrokinetic position and three-dimensional orientation control of a nanowire bioprobe in solution
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DOI：
10.1038/s41565-023-01439-7
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发表时间：
2023-07-27
影响因子：
38.3
通讯作者：
Fan，Donglei Emma
中科院分区：
文献类型：
--
作者：
Li，Huaizhi;Teal，Daniel;Fan，Donglei Emma
由于布朗运动效应，在溶液中精确操纵单个微米和纳米颗粒是具有挑战性的。因此，基于扫描探针的技术，如原子力显微镜，将颗粒附着在微杆上，使其能够用作纳米探针。在这里，我们展示了一个多功能的电动陷阱，同时控制二维位置的精度为20 nm和0.5°的三维取向的未拴系的纳米线，小到300 nm的长度，在光学显微镜下。该方法允许主动传输的纳米线与速度相关的精度达到90 nm，在2.7 μm s-1。它还允许它们在沿着沿着复杂轨迹移位期间同步三维对准和旋转。我们使用电动陷阱，准确地移动纳米探针，并稳定地将其定位在一个单一的细菌细胞的表面上，用于感测分泌的代谢物延长的时间。精确控制的操作支持开发用于组装、显微操作和具有亚细胞分辨率的生物测量的纳米机器人工具。
Owing to Brownian-motion effects, the precise manipulation of individual micro- and nanoparticles in solution is challenging. Therefore, scanning-probe-based techniques, such as atomic force microscopy, attach particles to cantilevers to enable their use as nanoprobes. Here we demonstrate a versatile electrokinetic trap that simultaneously controls the two-dimensional position with a precision of 20 nm and 0.5° in the three-dimensional orientation of an untethered nanowire, as small as 300 nm in length, under an optical microscope. The method permits the active transport of nanowires with a speed-dependent accuracy reaching 90 nm at 2.7 μm s–1. It also allows for their synchronous three-dimensional alignment and rotation during translocation along complex trajectories. We use the electrokinetic trap to accurately move a nanoprobe and stably position it on the surface of a single bacterial cell for sensing secreted metabolites for extended periods. The precision-controlled manipulation underpins developing nanorobotic tools for assembly, micromanipulation and biological measurements with subcellular resolution.