Differential Effects of Azithromycin, Doxycycline, and Cotrimoxazole in Ingested Blood on the Vectorial Capacity of Malaria Mosquitoes.
Differential Effects of Azithromycin, Doxycycline, and Cotrimoxazole in Ingested Blood on the Vectorial Capacity of Malaria Mosquitoes.
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DOI：
10.1093/ofid/ofw074
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发表时间：
2016-04
影响因子：
4.2
通讯作者：
Christophides GK
中科院分区：
文献类型：
--
作者：
Gendrin M;Yerbanga RS;Ouedraogo JB;Lefèvre T;Cohuet A;Christophides GK
背景。 疟疾媒介蚊子的肠道微生物群在吸血后会生长，并限制疟原虫感染。我们之前表明，摄入的血液中的青霉素和链霉素会影响细菌的生长，并对蚊子的生存和疟原虫的容许度产生积极影响。在这项研究中，我们检查了强力霉素、阿奇霉素和复方新诺明的作用。所有 3 种抗生素均用于大规模药物管理计划，并对细菌和不同阶段的疟原虫具有抗菌活性。方法。 评估了补充抗生素的血粉对蚊子微生物群、寿命和对恶性疟原虫的耐受性的影响。结果。 摄入 3 种抗生素中的任何一种都会显着影响蚊子微生物群。阿奇霉素降低了恶性疟原虫感染量和蚊子寿命，而高浓度的多西环素则增加了恶性疟原虫感染量。复方新诺明对感染强度产生负面影响，但对蚊子寿命没有可重复的影响。结论。 我们的数据表明，抗生素治疗对蚊子传播能力关键参数的总体影响是药物特异性的。阿奇霉素对疟疾传播的负面影响与当前消除疾病的努力是一致的，而在得出有关强力霉素的结论之前，还需要进行额外的、更大规模的研究。
Background. The gut microbiota of malaria vector mosquitoes grows after a blood meal and limits Plasmodium infection. We previously showed that penicillin and streptomycin in the ingested blood affect bacterial growth and positively impact mosquito survival and permissiveness to Plasmodium. In this study, we examine the effects of doxycycline, azithromycin, and co-trimoxazole. All 3 antibiotics are used in mass drug administration programs and have antimicrobial activities against bacteria and various stages of malaria parasites. Methods. The effects of blood meal supplementation with antibiotics on the mosquito microbiota, lifespan, and permissiveness to Plasmodium falciparum were assessed. Results. Ingestion of any of the 3 antibiotics significantly affected the mosquito microbiota. Azithromycin decreased P falciparum infection load and mosquito lifespan, whereas at high concentrations, doxycycline increased P falciparum infection load. Co-trimoxazole negatively impacted infection intensity but had no reproducible effect on mosquito lifespan. Conclusions. Our data suggest that the overall effect of antibiotic treatment on parameters critical for mosquito vectorial capacity is drug specific. The negative effect of azithromycin on malaria transmission is consistent with current efforts for disease elimination, whereas additional, larger scale investigations are required before conclusions can be drawn about doxycycline.