Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island
Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island
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负责精确整合和切除鸭疫里默氏菌基因组岛的整合酶的鉴定
DOI：
10.3389/fmicb.2019.02099
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发表时间：
2019-09
影响因子：
5.2
通讯作者：
Li Zili
中科院分区：
文献类型：
--
作者：
Wang Ying;Zhang Yang;Cui Yijie;Sun Zhijian;Zhou Zutao;Hu Sishun;Li Shaowen;Liu Mei;Meng Xianrong;Xiao Yuncai;Shi Deshi;Bi Dingren;Li Zili
鸭疫里默氏杆菌是一种革兰氏阴性致病菌，对家禽危害很大。然而，目前对鸭疫杆菌基因组岛(GIs)的研究还很少。本研究通过对鸭疫杆菌ATCC 11845的生物信息学分析，预测了一个10K的基因组岛，该基因广泛存在于其他禽疫杆菌基因组中。我们首次报道了鸭疫杆菌的基因组岛整合和切割功能。利用整合酶和53个碱基对的attP元件成功构建了整合载体。在鸭疫杆菌RA-ym染色体Arg-tRNA的53bpattB处发现了10K GI的整合子。我们鉴定了帮助鸭疫杆菌精确整合和切割的整合酶，并阐明了10K基因组岛整合和切割的分子机制。为基因的表达和互补菌株的构建提供了一种新的方法。
Riemerella anatipestifer is a Gram-negative, pathogenic bacterium, which is harmful to poultry. However, the genomic islands (GIs) in R. anatipestifer are not well-studied. In this study, a 10K genomic island was predicted by the bioinformatics analysis of R. anatipestifer ATCC 11845, which is widely found in other R. anatipestifer genomes. We had first reported the genomic island integration and excision function in R. anatipestifer. We successfully constructed the integration plasmid by using the integrase and 53 bp attP elements. The 10K GI was found integrated at the 53 bp attB located in the Arg-tRNA of the R. anatipestifer RA-YM chromosome. We identified an integrase that helped in the precise integration and excision in R. anatipestifer and elucidated the molecular mechanism of the 10K genomic island integration and excision. Furthermore, we provided a new method for the gene expression and construction of complementary strain.
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影响因子：
3.6
作者：
Mir-Sanchis, Ignacio;Martinez-Rubio, Roser;Penades, Jose R.
通讯作者：
Penades, Jose R.
Islandviewer 4：扩展了大规模数据集基因组岛的预测。
影响因子：
14.9
作者：
Bertelli C;Laird MR;Williams KP;Simon Fraser University Research Computing Group;Lau BY;Hoad G;Winsor GL;Brinkman FSL
通讯作者：
Brinkman FSL
影响因子：
14.9
作者：
J. Bergemann;K. Kühlcke;B. Fehse;I. Ratz;W. Ostertag;H. Lother
通讯作者：
J. Bergemann;K. Kühlcke;B. Fehse;I. Ratz;W. Ostertag;H. Lother
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
5.2
作者：
Wang Y;Lu T;Yin X;Zhou Z;Li S;Liu M;Hu S;Bi D;Li Z
通讯作者：
Li Z