PREPONTINE KNIFE CUT-INDUCED HYPERTHERMIA IN THE RAT - EFFECT OF CHEMICAL SYMPATHECTOMY AND SURGICAL DENERVATION OF BROWN ADIPOSE-TISSUE
PREPONTINE KNIFE CUT-INDUCED HYPERTHERMIA IN THE RAT - EFFECT OF CHEMICAL SYMPATHECTOMY AND SURGICAL DENERVATION OF BROWN ADIPOSE-TISSUE
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DOI：
10.1007/bf00580853
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发表时间：
1988-06-01
影响因子：
4.5
通讯作者：
GIRARDIER, L
中科院分区：
文献类型：
--
作者：
BENZI, RH;SHIBATA, M;GIRARDIER, L
研究褐色脂肪组织（BAT）去神经支配对术前刀切热疗的影响。先前的研究表明，由于BAT的显著激活，刀伤会引起3至4摄氏度的稳定高温。病变前肩胛间BAT （IBAT）温度低于结肠温度，但病变后几分钟温度梯度逆转，分数血流量增加12倍。对IBAT供血的5条神经进行双侧切开，既没有改变IBAT热反应的大小，也没有改变IBAT热反应的动力学。病变前，去神经组织的IBAT分数血流量是完整组织的15倍，病变后，尽管组织代谢活性急剧增加，但IBAT分数血流量几乎没有增加。化学交感神经切除术联合6-羟多巴胺可抑制高热反应。在稳态热疗期间静脉注射心得安或六甲溴铵导致IBAT温度迅速下降，并且在失神经和完整IBAT中IBAT和结肠温度之间的梯度逆转。去地帕明是一种去甲肾上腺素再摄取抑制剂，注射去地帕明会导致完整组织和去神经组织的温度升高，这是后者存在功能性残余神经支配的间接证据。这些结果表明，血管运动纤维在供应IBAT的肋间神经中运行，由于切断这些神经并没有阻止对组织的交感刺激，因此可以推断，进入IBAT的残留神经支配与血管一起传递源自外周结构的刺激信号。Moragas和Toran（1985）观察到无脑婴儿的BAT中有非常活跃的脂肪分解，并在这些发现的背景下进行了讨论。
The effect of brown adipose tissue (BAT) denervation on the prepontine knife cut-induced hyperthermia was stuided. The knife cut has earlier been shown to induce a steady hyperthermia of 3 to 4.degree.C, as a result of marked activation of the BAT. Before the lesion, the interscapular BAT (IBAT) temperature was lower than the colonic temperature, but the temperature gradient reversed a few minutes after the lesion and the fractional blood flow increased 12-fold. Bilateral sectioning of the 5 nerves supplying IBAT did not modify either the magnitude or the kinetics of the IBAT hyperthermic response. The IBAT fractional blood flow, which was 15 times higher in denervated than in intact tissue before the lesion, scarcely increased following the lesion despite the sharp increase in the tissue''s metabolic activity. Chemical sympathectomy with 6-hydroxydopamine suppressed the hyperthermic response. Propranolol or hexamethonium injected i.v. during the steady state hyperthermia resulted in a rapid drop in IBAT temperature and in a reversal of the gradient between IBAT and colonic temperature both in denervated and in intact IBAT. Injection of desipramine, an inhibitor of normadrenaline reuptake, resulted in itself in an increase of temperature in both intact and denervated tissue, which is circumstantial evidence for the presence of a functional residual innervation in the latter. These results indicate that vasomotor fibres run within intercostal nerves supplying IBAT and since severing of these nerves did not prevent the sympathetic stimulation of the tissue, it can be inferred that the residual innervation entering the IBAT along with the blood vessels is conveying stimulating signals originating in peripheral structures. The observation made by Moragas and Toran (1985) of a very active lipolysis in BAT from anencephalic infants are discussed in the context of these findings.