Improved method for computing potential doubling time from flow cytometric data.
Improved method for computing potential doubling time from flow cytometric data.
复制标题
根据流式细胞术数据计算潜在倍增时间的改进方法。
DOI：
10.1002/cyto.990110214
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Calkins,DP
中科院分区：
文献类型：
--
作者：
White,RA;Terry,NH;Meistrich,ML;Calkins,DP
Relative movement methods use the timed progression of the mean fluorescence of cells which have heen labeled with monoclonal antibodies against bromodeoxyuridine and displayed with bivariate flow cytometry according to DNA and label content to compute duration of DNA synthesis,Ts. The relative movement is the difference of the mean DNA fluorescence of the labeled undivided cells from theG1channel relative to the difference between theG1andG2Mchannels. In this communication, we show how to extend this method to compute the potential doubling time,Tpot, the time required for a population of cells to double, given quiescent cells but no cell loss. A quantityvis introduced that is a function of the fraction of labeled divided cells and the fraction of labeled undivided cells. We show thatvis independent of time and is equal to In(2)T8/Tpotso thatTpot(equal to In(2)T8/v) can be directly found from the information available in computing the relative movement. The method is applied to Chinese hamster ovary cells to demonstrate its utility.
