THE INHIBITORY ACTIONS OF HOUTTUYNIA CORDATA AQUEOUS EXTRACT ON DENGUE VIRUS AND DENGUE-INFECTED CELLS
THE INHIBITORY ACTIONS OF HOUTTUYNIA CORDATA AQUEOUS EXTRACT ON DENGUE VIRUS AND DENGUE-INFECTED CELLS
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DOI：
10.1111/j.1745-4514.2010.00514.x
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Sripanidkulchai, Bungorn
中科院分区：
文献类型：
--
作者：
Leardkamolkarn, Vijittra;Sirigulpanit, Wipawan;Sripanidkulchai, Bungorn
研究鱼腥草（H. cordata）水提物对16681型登革热病毒2型（DEN-2）的抗病毒活性。结果表明，与HepG2细胞孵育前后（10100 μ g/mL），可显著降低细胞内DEN-2 RNA的产生，并降低登革热蛋白的表达。在直接阻断模式下，提取物与DEN-2结合，有效剂量（EC50）为0.8 μ g/mL，对细胞内病毒RNA复制有较强的抑制作用。当浓度低至1040 μ g/mL时，灯心草提取物对感染的LLC-MK2细胞的病毒粒子释放也有保护作用。高效液相色谱分析表明，金丝桃苷是其主要的生物活性成分，可能具有一定的抑制作用。提取物也被证明对人体血细胞没有遗传毒性作用。
The antiviral activities of Houttuynia cordata (H. cordata) aqueous extract against dengue virus serotype 2 (DEN-2), strain 16681, were evaluated in this study. The results showed that pre- and post-incubation of H. cordata extract (10100 mu g/mL) with HepG2 cells significantly reduced intracellular DEN-2 RNA production correlating with the decrease in dengue protein expression. In the direct blocking mode, the extract bound with DEN-2 and strongly inhibited the intracellular viral RNA replication with an effective dose (EC50) of 0.8 mu g/mL. Concentrations as low as 1040 mu g/mL of H. cordata extract also exhibited protective effect on virion release from infected LLC-MK2 cells. High-performance liquid chromatography analysis of H. cordata extract indicated that hyperoside was the predominant bioactive compound, and was likely to play a role in this inhibition. The extract was also shown to have no genotoxic effect on human blood cells.