GENOME-SPECIFIC SCAR MARKERS HELP SOLVE TAXONOMY ISSUES: A CASE STUDY WITH SINAPIS ARVENSIS (BRASSICEAE, BRASSICACEAE)
GENOME-SPECIFIC SCAR MARKERS HELP SOLVE TAXONOMY ISSUES: A CASE STUDY WITH SINAPIS ARVENSIS (BRASSICEAE, BRASSICACEAE)
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DOI：
10.3732/ajb.1000422
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发表时间：
2011-03-01
影响因子：
3
通讯作者：
Khavkin, Emil E.
中科院分区：
文献类型：
--
作者：
Pankin, Artem A.;Khavkin, Emil E.
研究前提：传统的芸苔科（芸苔科）物种分类学和命名法不能反映其系统发育。由大量分子数据支持的种和属限制的修订似乎至关重要。方法与结果：利用从非编码序列和编码序列中提取的基因组特异性多态性，开发出14个Brassica B基因组特异性的序列扩增区（SCAR）标记。对6种u型三角型芸苔属植物的77份种质资源进行了验证，并对7种野生芸苔科植物的23份种质资源进行了交叉扩增试验。在所有b基因组的芸苔属植物和Sinapis arvensis中均发现了疤痕。结论：SCAR标记可用于判别芸苔属植物和南芥属植物的b基因组染色体，可靠地鉴定其b基因组物种及其自然杂交种。综合分子证据支持修改芸苔属和sinapi属界限的建议。
Premise of the study: Traditional taxonomy and nomenclature of Brassiceae (Brassicaceae) species do not reflect their phylogeny. Revision of the species and generic limits supported by extensive molecular data seems crucial.Methods and Results: Genome-specifi c polymorphisms extracted from non-coding and coding sequences were used to develop 14 sequence characterized amplified region (SCAR) markers specific for the Brassica B genome. These SCARs were verified against 77 accessions of six U-triangle Brassica species and used to screen 23 accessions of seven wild Brassiceae species to test for their cross-species amplification. SCARs were found in all B-genome Brassica species and also in Sinapis arvensis.Conclusions: SCAR markers can be employed for discerning B-genome chromosomes in Brassica species and S. arvensis to reliably identify B-genome species and their natural hybrids. The combined molecular evidence supports the suggestion to revise the generic limits of Brassica and Sinapis.