INCREASE IN CROSS-LINKING OF TYPE-I AND TYPE-III COLLAGENS ASSOCIATED WITH VOLUME-OVERLOAD HYPERTROPHY
INCREASE IN CROSS-LINKING OF TYPE-I AND TYPE-III COLLAGENS ASSOCIATED WITH VOLUME-OVERLOAD HYPERTROPHY
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DOI：
10.1161/01.res.63.2.399
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发表时间：
1988-08-01
影响因子：
20.1
通讯作者：
HARPER, E
中科院分区：
文献类型：
--
作者：
IIMOTO, DS;COVELL, JW;HARPER, E
从8只正常狗心和7只容量超负荷性肥厚的狗心中分离和鉴定了I、III、IV和V型胶原。容量超负荷性肥厚动物在左心室舒张末压力和僵硬增加时被处死。用溶解性能、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和酶联免疫吸附试验对胶原蛋白进行了表征。犬左室心肌胶原含量由正常心脏的32.4%降至肥厚心脏的15.0%。我们将此归因于I型和III型胶原的可萃取性降低，从正常心脏的199.5 mg I型胶原和76.4mgIII型胶原，下降到肥厚心脏的83.5mgI型胶原和26.4mgIII型胶原。对照组和动静脉分流术组的IV型和V型胶原含量基本不变，对照组平均为15.7 mg/g和32.1 mg/g，肥厚组平均为12.5 mg/g和28.9 mg/g。I型和III型胶原数量的减少可能反映了这两种胶原中更大程度的交联性。溴化氰多肽分析证实，大分子交联肽的含量从正常组的3.96%增加到肥厚组的8.88%。我们得出结论，I型和III型胶原的交联会在容量超负荷的肥厚中增加，这与舒张期硬度的增加有关。
Types I, III, IV, and V collagen were isolated and characterized from eight normal dog hearts and seven with volume-overload hypertrophy. Animals with volume-overload hypertrophy were killed at a time when left ventricular end-diastolic pressure and stiffness were increased. The collagens were characterized by solubility properties, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, and enzyme-linked immunosorbent assay. The percentage of collagen obtained from canine left ventricles was decreased from 32.4% in normal hearts to 15.0% in hypertrophied hearts. We attribute this to a diminution in the extractability of types I and III collagen, which fell from 199.5 mg type I/g collagen and 76.4 mg type III/g collagen in normal hearts to 83.5 mg type I/g collagen and 26.4 mg type III/g collagen in hypertrophied hearts. The amount of types IV and V collagen isolated remained constant in both the control and arteriovenous shunt hearts averaging 15.7 mg type IV/g collagen and 32.1 mg type V/g collagen in control hearts and 12.5 mg type IV/g collagen and 28.9 mg type V/g collagen in hypertrophied hearts. The reduction in quantity of types I and III collagen probably reflects a greater degree of cross-linking in these two types of collagen. Cyanogen bromide peptide analysis confirmed that there was an increase of high molecular weight cross-linked peptides from 3.96% in normal samples to 8.88% in hypertrophied samples. We conclude that cross-linking of types I and III collagen increases in volume-overload hypertrophy and that this is associated with a rise in diastolic stiffness.