Bioactive glass coatings affect the behavior of osteoblast-like cells
Bioactive glass coatings affect the behavior of osteoblast-like cells
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DOI：
10.1016/j.actbio.2007.02.011
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发表时间：
2007-09-01
影响因子：
9.7
通讯作者：
Tomsia, Antoni P.
中科院分区：
文献类型：
--
作者：
Foppiano, Silvia;Marshall, Sally J.;Tomsia, Antoni P.
采用搪瓷技术在钛合金（Ti6Al4V）表面制备了生物活性玻璃的功能梯度涂层。这些创新的涂层可能是等离子喷涂羟基磷灰石涂层植入物的替代品。先前我们确定模拟体液（SBF）预处理有助于稳定FGCs [Foppiano S et al.]。生物学报，2006-2(2):13342。这项工作的主要目的是评估预处理FGCs与MC3T3-E1.4小鼠成骨前细胞的体外细胞相容性。与未包被Ti6Al4V和组织培养聚苯乙烯（TCPS）相比，我们评估了细胞在FGCs上的粘附、增殖和矿化。细胞粘附没有差异，但增殖在所有材料上都有显著差异，在FGCs上最高，其次是TCPS和Ti6Al4V。定性和定量矿化分析表明，所有材料均发生矿化。矿化层释放的无机磷酸盐含量差异显著，TCPS最高，其次是FGC和未包覆Ti6Al4V。这项工作的第二个目的是通过实时逆转录聚合酶链反应来评估FGCs通过其溶解产物间接影响基因表达的能力。FGC溶出产物诱导Runx-2的表达增加了2倍，I型胶原的表达减少了20%。这些基因分别是成骨细胞分化和矿化的调节因子。这项研究的结果表明，预处理的FGCs具有细胞相容性，并表明未来的工作可能允许改变组成来诱导首选基因表达。(c) 2007材料学报Elsevier Ltd.出版。版权所有。
Functionally graded coatings (FGCs) of bioactive glass on titanium alloy (Ti6Al4V) were fabricated by the enameling technique. These innovative coatings may be an alternative to plasma-sprayed, hydroxyapatite-coated implants. Previously we determined that a preconditioning treatment in simulated body fluid (SBF) helped to stabilize FGCs [Foppiano S et al. Acta Biomater 2006-2(2):13342]. The primary goal of this work was to assess the in vitro cytocompatibility of preconditioned FGCs with MC3T3-E1.4 mouse pre-osteoblastic cells. We evaluated cell adhesion, proliferation and mineralization on FGCs in comparison to uncoated Ti6Al4V and tissue culture polystyrene (TCPS). No difference in cell adhesion was identified, whereas proliferation was significantly different on all materials, being highest on FGCs followed by TCPS and Ti6Al4V. Qualitative and quantitative mineralization assays indicated that mineralization occurred on all materials. The amount of inorganic phosphate released by the mineralizing layers was significantly different, being highest on TCPS, followed by FGC and uncoated Ti6Al4V. The secondary objective of this work was to assess the ability of the FGCs to affect gene expression, indirectly, by means of their dissolution products, which was assessed by real-time reverse-transcription polymerase chain reaction. The FGC dissolution products induced a 2-fold increase in the expression of Runx-2, and a 20%,) decrease in the expression of collagen type I with respect to TCPS extract. These genes are regulators of osteoblast differentiation and mineralization, respectively. The findings of this study indicate that preconditioned FGCs are cytocompatible and suggest that future work may allow composition changes to induce preferred gene expression. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.