Extracellular neurofibrillary tangles are immunopositive for the 40 carboxy-terminal sequence of beta-amyloid protein.
Extracellular neurofibrillary tangles are immunopositive for the 40 carboxy-terminal sequence of beta-amyloid protein.
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细胞外神经原纤维缠结对 β-淀粉样蛋白的 40 羧基末端序列呈免疫阳性。
DOI：
10.1097/00005072-199812000-00004
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发表时间：
1998
影响因子：
3.2
通讯作者：
P. Mcgeer
中科院分区：
文献类型：
--
作者：
C. Schwab;H. Akiyama;E. Mcgeer;P. Mcgeer
神经纤维缠结（NFT）在许多神经退行性疾病中形成。在阿尔茨海默病（AD）中，细胞内NFT（iNFT）与细胞外β-淀粉样蛋白（Abeta）沉积物一起沿着发展。关于NFT是否与Abeta相关的报告不一致。在这里，我们研究NFT和他们的直接关系，在AD，唐氏综合征和帕金森痴呆症的关岛复杂的情况下，与A β样片段，使用一组抗体，识别不同的表位的A β。在所有疾病中，以及在老年对照中，大多数细胞外NFT（eNFT）用识别Abeta的40个羧基末端的抗体染色，但不识别其他表位。这种染色在形态学上可与先前描述的Abeta阳性“缠结相关淀粉样蛋白沉积物”（TAAD）区分，TAAD围绕一些eNFT，并且对Abeta分子的所有表位是免疫阳性的。一些iNFT与42个羧基末端表位的抗体具有免疫反应性，并且在较小程度上与Abeta的中间部分和更多N末端表位的抗体具有免疫反应性。这些结果可能表明Abeta和NFT之间的直接相互作用，尽管不能排除与NFT相关的其他表位的二次沉积或交叉反应性。
Neurofibrillary tangles (NFTs) form in a number of neurodegenerative disorders. In Alzheimer disease (AD), intracellular NFTs (iNFTs) develop along with extracellular beta-amyloid (Abeta) deposits. Reports on whether NFTs have Abeta associated with them are inconsistent. Here we study NFTs and their direct relationship with Abeta-like fragments in cases of AD, Down Syndrome, and the parkinsonism-dementia complex of Guam, using a panel of antibodies which recognize different epitopes of Abeta. In all diseases, as well as in the aged controls, the majority of extracellular NFTs (eNFTs) are stained with antibodies recognizing the 40 carboxy-terminal of Abeta, but not other epitopes. Such staining is morphologically distinguishable from the previously described Abeta positive 'tangle associated amyloid deposits' (TAADs), which surround some eNFTs, and are immunopositive for all epitopes of the Abeta molecule. Some iNFTs are immunoreactive with antibodies to the 42 carboxy-terminal epitope, and, to a lesser extent, with antibodies to midportions and more N-terminal epitopes of Abeta. These results may indicate a direct interaction between Abeta and NFTs, although secondary deposition or crossreactivity with other epitopes associated with NFTs cannot be ruled out.