Impact of probable interaction of low temperature and ambient fine particulate matter on the function of rats alveolar macrophages
Impact of probable interaction of low temperature and ambient fine particulate matter on the function of rats alveolar macrophages
复制标题
低温与环境细颗粒物可能相互作用对大鼠肺泡巨噬细胞功能的影响
DOI：
10.1016/j.etap.2016.12.011
复制
发表时间：
2017-01-01
影响因子：
4.3
通讯作者：
Niu, Jingping
中科院分区：
文献类型：
--
作者：
Luo, Bin;Liu, Jiangtao;Niu, Jingping
本研究旨在探讨低温和大气细颗粒物（PM2.5）对大鼠肺泡巨噬细胞（AM）的可能相互作用。从大鼠BALF中分离出AM，在不同温度（18、24、30、37 ℃）下暴露于PM2.5（0、25、50、100 μ g/ml）8h。结果表明，随着温度的降低和PM2.5浓度的增加，AM的存活率和吞噬功能均下降，其中以18 ℃ PM2.5浓度最高（100 μ g/ml）组的毒性最强。PM2.5和低温均能促进肿瘤坏死因子α（TNF α）、巨噬细胞炎性蛋白1 α（MIP-1 α）和白细胞介素6（IL-6）的释放，但仅在MIP-1 α的产生上存在显著的交互作用。粒细胞-巨噬细胞集落刺激因子（GM-CSF）检测无明显变化。以上结果表明，这两种因素对大鼠AM都是有害的，低温可增加PM2.5对AM的毒性。(C)2016爱思唯尔B. V.保留所有权利。
The present study aimed to explore the probable interaction of low temperature and ambient fine particulate matter (PM2.5) on rat alveolar macrophages (AMs). AMs were separated from rat BALF and exposed to PM2.5 (0, 25, 50, 100 mu g/ml) under different temperature (18, 24, 30, 37 degrees C) for 8 h. Results indicated that viability and phagocytosis function of AMs decreased with the decline of temperature and the rise of PM2.5 dose, and the strongest toxicity was shown in the highest PM2.5 (100 mu g/ml) exposure group at 18 C. Both PM2.5 and lower temperature increased the releasing of tumor necrosis factor alpha (TNF alpha), macrophage inflammatory protein la (MIP-1 alpha) and interleukin-6 (IL-6), while significant interaction was only found in MIP-1 alpha production. No obvious change was found in granulocyte-macrophage colony stimulating factor (GM-CSF) detection. These results indicated that both the two factors are harmful to rat AMs and lower temperature could increase the toxicity of PM2.5 on the AMs. (C) 2016 Elsevier B.V. All rights reserved.