Autoregulation of swrAA and motility in Bacillus subtilis
Autoregulation of swrAA and motility in Bacillus subtilis
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DOI：
10.1128/jb.00455-08
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发表时间：
2008-08-01
影响因子：
3.2
通讯作者：
Galizzi, Alessandro
中科院分区：
文献类型：
--
作者：
Calvio, Cinzia;Osera, Cecilia;Galizzi, Alessandro
我们表明，转录的基因swrAA，所需的群集迁移在枯草芽孢杆菌，是由两个启动子驱动：一个sigD依赖性启动子和一个假定的sig，4依赖性启动子，这是在液体Luria-Bertani培养基中生长过程中无活性，并成为活跃的反应调节剂DegU的磷酸化形式的存在下或半固体表面上。由于sigD转录增强SwrAA，这一发现表明，swrA的表达是由一个正反馈回路控制。我们还表明，SwrAA在游泳和群集运动的积极作用是防止在携带删除的双组分系统degS-degU的菌株，这种效果是独立的swrAA转录。因此，DegU和SwrAA两者必须存在以在B中实现完全运动性。枯草杆菌。
We demonstrate that transcription of the gene swrAA, required for swarming migration in Bacillus subtilis, is driven by two promoters: a sigD-dependent promoter and a putative sig,4-dependent promoter, which is inactive during growth in liquid Luria-Bertani medium and becomes active in the presence of the phosphorylated form of the response regulator DegU or on semisolid surfaces. Since sigD transcription is enhanced by SwrAA, this finding reveals that swrA expression is controlled by a positive feedback loop. We also demonstrate that the positive action of SwrAA in swimming and swarming motility is prevented in strains carrying a deletion of the two-component system degS-degU and that this effect is independent of swrAA transcription. Therefore, both DegU and SwrAA must be present to achieve full motility in B. subtilis.