Bioinformatics Applications Note Arrayqualitymetrics—a Bioconductor Package for Quality Assessment of Microarray Data
Bioinformatics Applications Note Arrayqualitymetrics—a Bioconductor Package for Quality Assessment of Microarray Data
复制标题
DOI：
--
复制
发表时间：
--
影响因子：
14.9
通讯作者：
Audrey Kauffmann;R. Gentleman;W. Huber
中科院分区：
文献类型：
--
作者：
Audrey Kauffmann;R. Gentleman;W. Huber
数据质量的评估是微阵列分析中的一个主要问题。ArrayQualityMetrics是一个BioConductor包，它提供了一份报告，其中包含一种或两种颜色微阵列数据的诊断曲线图。质量指标评估重复性，识别明显的离群点阵列，并计算信噪比的测量。该工具可处理大多数当前的微阵列技术，可用于自动化分析管道或自动生成报告，也可供个人使用。原则上，对质量的诊断仍然是一个依赖于背景的判断，但我们的工具提供了强大的、自动化的、客观的和全面的工具，作为决策的基础。可获得性：arrayQualityMetrics是一个免费的开放源码包，受LGPL许可，可从BioConductor项目www.BioConductor.org获得。该程序包随附了用户指南和示例。ArrayQualityMetrics生成的超文本标记语言报告的一些例子可以在http://www.microarray-quality.org补充信息：补充数据在生物信息学在线上找到。
The assessment of data quality is a major concern in microarray analysis. arrayQualityMetrics is a Bioconductor package that provides a report with diagnostic plots for one or two colour microarray data. The quality metrics assess reproducibility, identify apparent outlier arrays and compute measures of signal-to-noise ratio. The tool handles most current microarray technologies and is amenable to use in automated analysis pipelines or for automatic report generation, as well as for use by individuals. The diagnosis of quality remains, in principle, a context-dependent judgement, but our tool provides powerful, automated, objective and comprehensive instruments on which to base a decision. Availability: arrayQualityMetrics is a free and open source package, under LGPL license, available from the Bioconductor project at www.bioconductor.org. A users guide and examples are provided with the package. Some examples of HTML reports generated by arrayQualityMetrics can be found at http://www.microarray-quality.org Supplementary information: Supplementary data are available at Bioinformatics online.