Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
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DOI：
10.1007/s00343-009-9157-4
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发表时间：
2009-11
影响因子：
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通讯作者：
J. Niu;Guangce Wang;F. Lü;Bai-cheng Zhou;G. Peng
中科院分区：
文献类型：
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作者：
J. Niu;Guangce Wang;F. Lü;Bai-cheng Zhou;G. Peng
一组多核海藻不同于高等植物，其全能性依赖于完整的细胞（或原生质体）。相反，这种水母能够在海水中聚集其挤出的原生质并产生新的成熟个体。据认为，凝集素在聚集过程中起关键作用。我们纯化的凝集素与细胞器的聚集在苔藓hypnoides。凝集素是Ca。27 kDa，pI在pH 5和pH 6之间。不含糖，表明凝集素不是糖蛋白。凝集素的血凝活性（HA）不依赖于二价阳离子的存在下，并抑制N-乙酰半乳糖胺，N-乙酰葡糖胺，和糖蛋白牛颌下粘蛋白。凝集素优先凝集革兰氏阴性菌。该凝集素的HA在pH 4至pH 10之间是稳定的。通过加入凝集素溶液（0.5 mg ml-1）使细胞质外的细胞器凝集。我们的研究结果表明，再生B。hypnoides是由这种凝集素介导的。我们还表明，在天然海水（pH 8.0）中，细胞器聚集体的形成被尼日利亚菌素抑制。鉴于尼日利亚菌素消散质子梯度穿过膜，我们假设细胞器的聚集是质子梯度依赖性的。
A group of coenocytic marine algae differs from higher plants, whose totipotency depends on an intact cell (or protoplast). Instead, this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine, N-Acetylglucosamine, and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml−1). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane, we hypothesize that the aggregation of cell organelles was proton-gradient dependent.