INTERACTION OF MURINE PERITONEAL LEUKOCYTES AND MESOTHELIAL CELLS - IN-VITRO MODEL SYSTEM TO SURVEY CELLULAR EVENTS ON SEROSAL MEMBRANES DURING INFLAMMATION
INTERACTION OF MURINE PERITONEAL LEUKOCYTES AND MESOTHELIAL CELLS - IN-VITRO MODEL SYSTEM TO SURVEY CELLULAR EVENTS ON SEROSAL MEMBRANES DURING INFLAMMATION
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DOI：
10.1006/clin.1995.1076
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发表时间：
1995-06-01
期刊：
影响因子：
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通讯作者：
YOSHIDA, T
中科院分区：
文献类型：
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作者：
MULLER, J;YOSHIDA, T
所有浆膜腔，包括腹膜，都衬有单层鳞状间皮。建立了小鼠间皮细胞的原代培养体系，研究了间皮细胞与腹腔白细胞的相互作用，通过细胞角蛋白和波形蛋白的表达来确定间皮细胞的特性。间皮细胞表达ICAM-1和CD 44分子。TNF-α（100 U/ml）可显著增强ICAM-1的表达，但不增强CD 44的表达。我们还研究了转化生长因子TGF-α（20 ng/ml）和TGF-β（2 ng/ml）以及表皮生长因子（20 ng/ml）的可能影响。这些因素没有发现在体外调节ICAM-1或CD 44的表达。在共培养实验中，未刺激的间皮细胞几乎不粘附的居民和引发腹膜单核白细胞数小时。TNF-α或EGF预处理的间皮细胞大大增强了它们对腹膜单核白细胞的粘附亲和力，而TGF-β预处理甚至降低了低的基础粘附。延长共培养3周导致间皮表面上的驻留和诱导的单核细胞/巨噬细胞的显著增殖和分化，EGF刺激间皮细胞产生巨噬细胞集落刺激活性（M-CSA），抗M-CSF单克隆抗体证实M-CSA主要来源于M-CSF;残余的M-CSA不是由GM-CSF形成的。传代几代后，间皮细胞开始自发产生M-CSA。(C)出版社：Academic Press
All serosal cavities including peritoneum are lined with a simple squamous mesothelium. Primary culture of murine mesothelial cells has been established to study their cellular interactions with peritoneal leukocytes, The mesothelial character was determined by the cytokeratin and vimentin expression. The mesothelial cells expressed ICAM-1 and CD44 molecules. The expression of ICAM-1, but not CD44, was significantly enhanced by the treatment with TNF-alpha (100 U/ml). We have also investigated possible in fluence of transforming growth factors, TGF-alpha (20 ng/ml) and TGF-beta (2 ng/ml), and epidermal growth factor (20 ng/ml). These factors were not found to modulate ICAM-1 or CD44 expression in vitro. During coculture experiments unstimulated mesothelial cells were almost nonadherent for both resident and elicited peritoneal mononuclear leukocytes for several hours. TNF-alpha or EGF pretreatment of mesothelial cells greatly enhanced their adhesive affinity to peritoneal mononuclear leukocytes, while TGF-beta pretreatment even reduced the low basal adhesion, Prolonged co-culture for 3 weeks resulted in remarkable proliferation and differentiation of both resident and elicited monocytes/macrophages on the mesothelial surface, The stimulation of mesothelial cell culture with EGF resulted in the macrophage colony-stimulating activity (M-CSA) production, M-CSA was mainly due to M-CSF as confirmed with anti M-CSF monoclonal antibody; the residual M-CSA was not formed by GM-CSF. After several passages the mesothelial cells started to produce M-CSA spontaneously. (C) 1995 Academic Press, Inc.