Urinary platelet factor four (Pf4) levels in mesangial IgA glomerulonephritis and thin basement membrane disease.
Urinary platelet factor four (Pf4) levels in mesangial IgA glomerulonephritis and thin basement membrane disease.
复制标题
系膜 IgA 肾炎和薄基底膜疾病中的尿血小板因子四 (Pf4) 水平。
作者：
K. Taira;T. Hewitson;P. Kincaid‐smith
本研究的目的是评估系膜伊加肾小球肾炎（IgAGN）和薄基底膜病（TBMD）患者尿液中血小板第四因子（Pf 4）排泄的意义，并确定Pf 4是否是区分这些疾病的有用参数。采用酶联免疫吸附试验（ELISA）测定IgAGN（n = 80）、TBMD（n = 37）、膜性肾病（n = 12）、微小病变型肾病综合征（n = 3）、新月体肾炎（n = 6）和健康对照组（n = 20）尿中Pf 4浓度。并与尿常规及肾功能进行比较。采用免疫过氧化物酶法检测尿沉渣中血小板的存在。80例IgAGN患者中有35例检测到尿Pf 4（中位数0.15，范围0.07-2.5 ng/ml，n = 35），37例TBMD患者中仅2例检测到尿Pf 4（p <0.005），所有CGN患者均检测到尿Pf 4，MN、MCNS或对照组患者均未检测到尿Pf 4。IgAGN患者尿Pf 4水平与红细胞计数呈正相关（r = 0.876，p <0.001），但TBMD患者尿Pf 4水平与红细胞计数不相关。在两组中，pf 4与蛋白尿或血浆肌酐无关。15例IgAGN患者中10例尿中检出血小板，而9例TBMD患者中仅1例尿中检出血小板。24例患者尿红细胞计数均在10万/ml以上。（250字处删节）
The aim of this study was to assess the significance of platelet factor four (Pf4) excretion in the urine of patients with mesangial IgA glomerulonephritis (IgAGN) and thin basement membrane disease (TBMD), and ascertain if Pf4 is a useful parameter to distinguish these diseases. The concentration of Pf4 in urine (Pf4) was determined by an enzyme linked immunoabsorbent assay (ELISA) in patients with IgAGN (n = 80), TBMD (n = 37), membranous nephropathy (MN) (n = 12), minimal change nephrotic syndrome (MCNS) (n = 3), crescentic glomerulonephritis (CGN) (n = 6) and in healthy controls (n = 20), and then compared with urinalysis and renal function. An immunoperoxidase method was used to examine urine sediments for the presence of platelets. Urinary Pf4 was detected in 35 out of 80 patients with IgAGN (median 0.15, range 0.07-2.5 ng/ml, n = 35), in only 2 out of 37 patients with TBMD (p less than 0.005), in all patients with CGN and in no patients from MN, MCNS or control groups. A positive correlation between urinary Pf4 levels and red blood cell counts was observed in patients with IgAGN (r = 0.876, p less than 0.001), but not in TBMD. Pf4 did not correlate with proteinuria or plasma creatinine in either group. Platelets were detected in urine in 10 out of 15 patients with IgAGN but in only 1 out of 9 patients with TBMD. Urinary red blood cell counts in all of these 24 patients were over 100,000/ml.(ABSTRACT TRUNCATED AT 250 WORDS)