Antioxidant Activity of Vitamin E and Trolox: Understanding of the Factors that Govern Lipid Peroxidation Studies In Vitro
Antioxidant Activity of Vitamin E and Trolox: Understanding of the Factors that Govern Lipid Peroxidation Studies In Vitro
复制标题
DOI：
10.1007/s11483-009-9129-4
复制
发表时间：
2009-12-01
期刊：
影响因子：
3
通讯作者：
Reis, Salette
中科院分区：
文献类型：
--
作者：
Lucio, Marlene;Nunes, Claudia;Reis, Salette
脂质的过氧化是一个重要的研究课题，因为有证据表明，过氧自由基和脂质过氧化产物可能参与了含脂质食品加工和储存过程中引发变质反应的化合物的毒性。鉴于膳食复合维生素E及其水溶性类似物Trolox在含脂质食品研究中的抗氧化作用的重要性，需要更具体地确定它们如何以及在何处在脂质膜中发挥其抗氧化活性。在这项研究中，单层脂质体的磷脂酰胆碱被用作膜模拟系统，以估计维生素E和Trolox的抗氧化性能，并建立它们之间的相互作用与膜和随之而来的抗氧化活性的关系。脂质过氧化反应由脂质和水介质中的过氧自由基（ROOaEuro cent）通过偶氮化合物的热分解引发，并通过荧光探针二苯基己三烯丙酸的荧光强度衰减进行评估。所获得的结果表明，膜脂质过氧化不仅与所研究的化合物的清除特性有关，而且与它们与脂质双层相互作用的能力有关，因此脂质体提供了额外的信息，目前从在水性缓冲介质中进行的测定中获得的信息。
Peroxidation of lipids is of significant interest owing to the evidence that peroxyl radicals and products of lipid peroxidation may be involved in the toxicity of compounds initiating a deteriorative reaction in the processing and storage of lipid-containing foods. In view of the significance of the antioxidant role of the dietary compound vitamin E and its water-soluble analogue Trolox in research of lipid-containing foods, it is desirable to determine more specifically how and where they operate its antioxidant activity in lipid membranes. In this study, unilamellar liposomes of phosphatidylcholine were used as membrane mimetic systems to estimate the antioxidant properties of vitamin E and Trolox and establish a relationship between their interactions with the membrane and their consequent antioxidant activity. Lipid peroxidation was initiated by the peroxyl radical (ROOaEuro cent) in lipid and aqueous media by the thermal decomposition of azocompounds and was assessed by the fluorescence intensity decay of the fluorescent probe diphenylhexatriene propionic acid. Results obtained showed that membrane lipoperoxidation is related not only to the scavenging characteristics of the compounds studied but also to their ability to interact with the lipid bilayers, and consequently liposomes provide additional information to that obtained currently from assays performed in aqueous buffer media.