Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue
Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue
复制标题
DOI：
10.1117/1.2892687
复制
发表时间：
2008-03-01
影响因子：
3.5
通讯作者：
Goldstein, Steven A.
中科院分区：
文献类型：
--
作者：
Schulmerich, Matthew V.;Cole, Jacqueline H.;Goldstein, Steven A.
研究了甘油透光对4只小鼠左、右侧胫骨经皮获取的骨组织拉曼光谱的影响。从每个肢体获得多次经皮测量；然后应用甘油作为光学清洗剂，并进行额外的经皮测量。甘油降低了原始光谱中的噪音(p=0.0037)，并显著改善了在骨骼光谱的低信噪比区域中恢复的骨骼因子与暴露的骨骼测量之间的相互关系(p=0.0245)。(C)2008年光学仪器工程师学会。
The effect of optical clearing with glycerol on the Raman spectra of bone tissue acquired transcutaneously on right and left tibiae from four mice is studied. Multiple transcutaneous measurements are obtained from each limb; glycerol is then applied as an optical clearing agent, and additional transcutaneous measurements are taken. Glycerol reduces the noise in the raw spectra (p = 0.0037) and significantly improves the cross-correlation between the recovered bone factor and the exposed bone measurement in a low signal-to-noise region of the bone spectra (p=0.0245). (C) 2008 Society of Photo-Optical Instrumentation Engineers.