GENERATION OF MEMORY B-CELLS AND PLASMA-CELLS IN-VITRO
GENERATION OF MEMORY B-CELLS AND PLASMA-CELLS IN-VITRO
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DOI：
10.1126/science.7537388
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发表时间：
1995-05-05
期刊：
影响因子：
56.9
通讯作者：
LIU, YJ
中科院分区：
文献类型：
--
作者：
ARPIN, C;DECHANET, J;LIU, YJ
在生殖中心B细胞经历体细胞突变和抗原选择后，它们变成记忆B细胞或浆细胞，但控制进入任一途径的信号要求尚不清楚。当纯化的人生发中心细胞与白细胞介素-2、白细胞介素-10和表达CD 40配体的细胞一起培养时，产生了具有记忆B细胞特征的细胞。从系统中去除CD 40配体导致生发中心B细胞终末分化为具有浆细胞特征的细胞。这些结果表明，CD 40配体指导生发中心B细胞向记忆B细胞而不是向浆细胞分化。
After germinal center B cells undergo somatic mutation and antigen selection, they become either memory B cells or plasma cells, but the signal requirements that control entry into either pathway have been unclear. When purified human germinal center cells were cultured with interleukin-2, interleukin-10, and cells expressing CD40 ligand, cells with characteristics of memory B cells were generated. Removal of CD40 ligand from the system resulted in terminal differentiation of germinal center B cells into cells with the characteristics of plasma cells. These results indicate that CD40 ligand directs the differentiation of germinal center B cells toward memory B cells rather than toward plasma cells.