TEMPy2: a Python library with improved 3D electron microscopy density-fitting and validation workflows.
TEMPy2: a Python library with improved 3D electron microscopy density-fitting and validation workflows.
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DOI：
10.1107/s2059798320014928
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发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Topf M
中科院分区：
文献类型：
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作者：
Cragnolini T;Sahota H;Joseph AP;Sweeney A;Malhotra S;Vasishtan D;Topf M
TEMPy2是temy软件包的更新，用于处理、优化和评估低温电镜图及其结构。通过低温电子显微镜进行分子复合物的结构测定需要对最初获得的图像数据进行大量且通常复杂的处理。在这里，TEMPy2是TEMPy包的更新，用于处理、优化和评估低温电镜图和适合于它们的结构。描述了执行这些任务的新的优化例程，全面的自动化检查和工作流程。
TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is presented. Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.