DIFFERENTIATION OF CARBOHYDRATES BY ANTHRONE REACTION RATE AND COLOR INTENSITY

DIFFERENTIATION OF CARBOHYDRATES BY ANTHRONE REACTION RATE AND COLOR INTENSITY
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DOI:
10.1021/ac60070a014
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发表时间:
1952-01-01
影响因子:
7.4
通讯作者:
KOEHLER, LH
KOEHLER, LH
中科院分区:
化学1区
文献类型:
--
作者:
KOEHLER, LH

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本研究的目的是使蒽酮-硫酸糖显色反应适用于天然多糖复合物的研究。已知碳水化合物的行为首先被确立为比较的基础。已知的糖类型显示出独特的反应速率和强度;在98 ℃下加热5至10分钟的特征最大值时,己醛糖的读数为每100微克110至165 Klett单位;在98 ℃下加热1至2分钟,己酮糖的读数为每100微克220至270单位。
The aim of this investigation was to adapt the anthrone-sulfuric acid carbohydrate colorreaction to the study of native polysaccharide complexes. The behavior of known carbohydrates was first established as the basis of comparison. Known sugar types showed distinctive reaction rates and intensities; aldohexoses gave readings of 110 to 165 Klett units per 100 micrograms at their characteristic maximum of 5 to 10 minutes of heating at 98; ketohexoses, 220 to 270 units per 100 micro-grams at their maximum, 1 to 2 minutes at 98.